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TitleThe structure of purified kinetochores reveals multiple microtubule-attachment sites.
Journal, issue, pagesNat Struct Mol Biol, Vol. 19, Issue 9, Page 925-929, Year 2012
Publish dateAug 12, 2012
AuthorsShane Gonen / Bungo Akiyoshi / Matthew G Iadanza / Dan Shi / Nicole Duggan / Sue Biggins / Tamir Gonen /
PubMed AbstractChromosomes must be accurately partitioned to daughter cells to prevent aneuploidy, a hallmark of many tumors and birth defects. Kinetochores are the macromolecular machines that segregate ...Chromosomes must be accurately partitioned to daughter cells to prevent aneuploidy, a hallmark of many tumors and birth defects. Kinetochores are the macromolecular machines that segregate chromosomes by maintaining load-bearing attachments to the dynamic tips of microtubules. Here, we present the structure of isolated budding-yeast kinetochore particles, as visualized by EM and electron tomography of negatively stained preparations. The kinetochore appears as an ~126-nm particle containing a large central hub surrounded by multiple outer globular domains. In the presence of microtubules, some particles also have a ring that encircles the microtubule. Our data, showing that kinetochores bind to microtubules via multivalent attachments, lay the foundation to uncover the key mechanical and regulatory mechanisms by which kinetochores control chromosome segregation and cell division.
External linksNat Struct Mol Biol / PubMed:22885327 / PubMed Central
MethodsEM (tomography)
Structure data

EMDB-2153:
Electron tomography of Saccharomyces cerevisiae kinetochore fragment on a taxol stabilized microtubule
Method: EM (tomography)

EMDB-2154:
Electron tomography of Saccharomyces cerevisiae kinetochore fragment on a taxol stabilized microtubule
Method: EM (tomography)

Source
  • Saccharomyces cerevisiae (brewer's yeast)

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