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TitleHigh-Throughput Cryo-EM Enabled by User-Free Preprocessing Routines.
Journal, issue, pagesStructure, Vol. 28, Issue 7, Page 858-869.e3, Year 2020
Publish dateJul 7, 2020
AuthorsYilai Li / Jennifer N Cash / John J G Tesmer / Michael A Cianfrocco /
PubMed AbstractSingle-particle cryoelectron microscopy (cryo-EM) continues to grow into a mainstream structural biology technique. Recent developments in data collection strategies alongside new sample preparation ...Single-particle cryoelectron microscopy (cryo-EM) continues to grow into a mainstream structural biology technique. Recent developments in data collection strategies alongside new sample preparation devices herald a future where users will collect multiple datasets per microscope session. To make cryo-EM data processing more automatic and user-friendly, we have developed an automatic pipeline for cryo-EM data preprocessing and assessment using a combination of deep-learning and image-analysis tools. We have verified the performance of this pipeline on a number of datasets and extended its scope to include sample screening by the user-free assessment of the qualities of a series of datasets under different conditions. We propose that our workflow provides a decision-free solution for cryo-EM, making data preprocessing more generalized and robust in the high-throughput era as well as more convenient for users from a range of backgrounds.
External linksStructure / PubMed:32294468 / PubMed Central
MethodsEM (single particle)
Resolution3.0 - 3.2 Å
Structure data

EMDB-21490:
Influenza hemagglutinin trimer preprocessed with automatic workflow
Method: EM (single particle) / Resolution: 3.2 Å

EMDB-21491:
Thermoplasma acidophilum 20S proteasome cryo-EM structure using automatic preprocessing workflow
Method: EM (single particle) / Resolution: 3.0 Å

EMDB-21492:
Rabbit muscle aldolase cryo-EM structure using automatic preprocessing workflow
Method: EM (single particle) / Resolution: 3.2 Å

Source
  • unidentified (others)
  • Thermoplasma acidophilum (acidophilic)
  • Oryctolagus cuniculus (rabbit)

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