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TitleGasdermin D pore structure reveals preferential release of mature interleukin-1.
Journal, issue, pagesNature, Vol. 593, Issue 7860, Page 607-611, Year 2021
Publish dateApr 21, 2021
AuthorsShiyu Xia / Zhibin Zhang / Venkat Giri Magupalli / Juan Lorenzo Pablo / Ying Dong / Setu M Vora / Longfei Wang / Tian-Min Fu / Matthew P Jacobson / Anna Greka / Judy Lieberman / Jianbin Ruan / Hao Wu /
PubMed AbstractAs organelles of the innate immune system, inflammasomes activate caspase-1 and other inflammatory caspases that cleave gasdermin D (GSDMD). Caspase-1 also cleaves inactive precursors of the ...As organelles of the innate immune system, inflammasomes activate caspase-1 and other inflammatory caspases that cleave gasdermin D (GSDMD). Caspase-1 also cleaves inactive precursors of the interleukin (IL)-1 family to generate mature cytokines such as IL-1β and IL-18. Cleaved GSDMD forms transmembrane pores to enable the release of IL-1 and to drive cell lysis through pyroptosis. Here we report cryo-electron microscopy structures of the pore and the prepore of GSDMD. These structures reveal the different conformations of the two states, as well as extensive membrane-binding elements including a hydrophobic anchor and three positively charged patches. The GSDMD pore conduit is predominantly negatively charged. By contrast, IL-1 precursors have an acidic domain that is proteolytically removed by caspase-1. When permeabilized by GSDMD pores, unlysed liposomes release positively charged and neutral cargoes faster than negatively charged cargoes of similar sizes, and the pores favour the passage of IL-1β and IL-18 over that of their precursors. Consistent with these findings, living-but not pyroptotic-macrophages preferentially release mature IL-1β upon perforation by GSDMD. Mutation of the acidic residues of GSDMD compromises this preference, hindering intracellular retention of the precursor and secretion of the mature cytokine. The GSDMD pore therefore mediates IL-1 release by electrostatic filtering, which suggests the importance of charge in addition to size in the transport of cargoes across this large channel.
External linksNature / PubMed:33883744 / PubMed Central
MethodsEM (single particle)
Resolution3.9 - 6.9 Å
Structure data

21160

6vfe

EMDB-21160, PDB-6vfe:
Gasdermin D pore
Method: EM (single particle) / Resolution: 3.9 Å

EMDB-21161:
Pore-forming protein
Method: EM (single particle) / Resolution: 6.9 Å

Source
  • homo sapiens (human)
KeywordsLIPID BINDING PROTEIN / Pore-forming protein

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