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TitleStructural basis of assembly chaperone- mediated snRNP formation.
Journal, issue, pagesMol Cell, Vol. 49, Issue 4, Page 692-703, Year 2013
Publish dateFeb 21, 2013
AuthorsClemens Grimm / Ashwin Chari / Jann-Patrick Pelz / Jochen Kuper / Caroline Kisker / Kay Diederichs / Holger Stark / Hermann Schindelin / Utz Fischer /
PubMed AbstractSmall nuclear ribonucleoproteins (snRNPs) represent key constituents of major and minor spliceosomes. snRNPs contain a common core, composed of seven Sm proteins bound to snRNA, which forms in a step- ...Small nuclear ribonucleoproteins (snRNPs) represent key constituents of major and minor spliceosomes. snRNPs contain a common core, composed of seven Sm proteins bound to snRNA, which forms in a step-wise and factor-mediated reaction. The assembly chaperone pICln initially mediates the formation of an otherwise unstable pentameric Sm protein unit. This so-called 6S complex docks subsequently onto the SMN complex, which removes pICln and enables the transfer of pre-assembled Sm proteins onto snRNA. X-ray crystallography and electron microscopy was used to investigate the structural basis of snRNP assembly. The 6S complex structure identifies pICln as an Sm protein mimic, which enables the topological organization of the Sm pentamer in a closed ring. A second structure of 6S bound to the SMN complex components SMN and Gemin2 uncovers a plausible mechanism of pICln elimination and Sm protein activation for snRNA binding. Our studies reveal how assembly factors facilitate formation of RNA-protein complexes in vivo.
External linksMol Cell / PubMed:23333303
MethodsEM (single particle) / X-ray diffraction
Resolution1.898 - 20.0 Å
Structure data

EMDB-2102:
Structure of a stalled transfer intermediate of Sm proteins from the assembly chaperone pICln to the SMN-complex
Method: EM (single particle) / Resolution: 20.0 Å

PDB-4f7u:
The 6S snRNP assembly intermediate
Method: X-RAY DIFFRACTION / Resolution: 1.898 Å

PDB-4v98:
The 8S snRNP Assembly Intermediate
Method: X-RAY DIFFRACTION / Resolution: 3.1 Å

Chemicals

ChemComp-P6G:
HEXAETHYLENE GLYCOL / precipitant*YM / Polyethylene glycol

ChemComp-HOH:
WATER / Water

ChemComp-SO4:
SULFATE ION / Sulfate

Source
  • homo sapiens (human)
  • mus musculus (house mouse)
  • xenopus laevis (African clawed frog)
  • drosophila melanogaster (fruit fly)
KeywordsSPLICING / Assembly Machine / Complex / Assembly Machinery

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