EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Gating of human TRPV3 in a lipid bilayer.
ジャーナル・号・ページ	Nat Struct Mol Biol, Vol. 27, Issue 7, Page 635-644, Year 2020
掲載日	2020年6月22日
著者	Zengqin Deng / Grigory Maksaev / Michael Rau / Zili Xie / Hongzhen Hu / James A J Fitzpatrick / Peng Yuan /
PubMed 要旨	The transient receptor potential cation channel subfamily V member 3 (TRPV3) channel plays a critical role in skin physiology, and mutations in TRPV3 result in the development of a congenital skin ...The transient receptor potential cation channel subfamily V member 3 (TRPV3) channel plays a critical role in skin physiology, and mutations in TRPV3 result in the development of a congenital skin disorder, Olmsted syndrome. Here we describe multiple cryo-electron microscopy structures of human TRPV3 reconstituted into lipid nanodiscs, representing distinct functional states during the gating cycle. The ligand-free, closed conformation reveals well-ordered lipids interacting with the channel and two physical constrictions along the ion-conduction pore involving both the extracellular selectivity filter and intracellular helix bundle crossing. Both the selectivity filter and bundle crossing expand upon activation, accompanied by substantial structural rearrangements at the cytoplasmic intersubunit interface. Transition to the inactivated state involves a secondary structure change of the pore-lining helix, which contains a π-helical segment in the closed and open conformations, but becomes entirely α-helical upon inactivation. Together with electrophysiological characterization, structures of TRPV3 in a lipid membrane environment provide unique insights into channel activation and inactivation mechanisms.
リンク	Nat Struct Mol Biol / PubMed:32572252 / PubMed Central
手法	EM (単粒子)
解像度	3.1 - 4.33 Å
構造データ	20917 6uw4 EMDB-20917, PDB-6uw4: Cryo-EM structure of human TRPV3 determined in lipid nanodisc 手法: EM (単粒子) / 解像度: 3.1 Å 20918 6uw6 EMDB-20918, PDB-6uw6: Cryo-EM structure of the human TRPV3 K169A mutant determined in lipid nanodisc 手法: EM (単粒子) / 解像度: 3.66 Å 20919 6uw8 EMDB-20919, PDB-6uw8: Cryo-EM structure of the human TRPV3 K169A mutant briefly exposed to 2-APB for 3 minutes, determined in lipid nanodisc 手法: EM (単粒子) / 解像度: 4.02 Å 20920 6uw9 EMDB-20920, PDB-6uw9: Cryo-EM structure of the human TRPV3 K169A mutant in the presence of 2-APB, determined in lipid nanodisc 手法: EM (単粒子) / 解像度: 4.33 Å
化合物	ChemComp-6OU: [(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{Z})-octadec-9-enoate / POPE / リン脂質YM ChemComp-NA*: Unknown entry / ナトリウムカチオン
由来	homo sapiens (ヒト)
キーワード	MEMBRANE PROTEIN (膜タンパク質) / ion channel (イオンチャネル) / TRPV3 / ion channel TRPV3