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TitleStructural and Proteomic Characterization of the Initiation of Giant Virus Infection.
Journal, issue, pagesCell, Vol. 181, Issue 5, Page 1046-11061.e6, Year 2020
Publish dateMay 28, 2020
AuthorsJason R Schrad / Jônatas S Abrahão / Juliana R Cortines / Kristin N Parent /
PubMed AbstractSince their discovery, giant viruses have expanded our understanding of the principles of virology. Due to their gargantuan size and complexity, little is known about the life cycles of these viruses. ...Since their discovery, giant viruses have expanded our understanding of the principles of virology. Due to their gargantuan size and complexity, little is known about the life cycles of these viruses. To answer outstanding questions regarding giant virus infection mechanisms, we set out to determine biomolecular conditions that promote giant virus genome release. We generated four infection intermediates in Samba virus (Mimivirus genus, lineage A) as visualized by cryoelectron microscopy (cryo-EM), cryoelectron tomography (cryo-ET), and scanning electron microscopy (SEM). Each of these four intermediates reflects similar morphology to a stage that occurs in vivo. We show that these genome release stages are conserved in other mimiviruses. Finally, we identified proteins that are released from Samba and newly discovered Tupanvirus through differential mass spectrometry. Our work revealed the molecular forces that trigger infection are conserved among disparate giant viruses. This study is also the first to identify specific proteins released during the initial stages of giant virus infection.
External linksCell / PubMed:32392465 / PubMed Central
MethodsEM (tomography)
Structure data

EMDB-20745:
Low pH and high temperature-treated Samba virus particles
Method: EM (tomography)

EMDB-20746:
Low pH and High Temperature-Treated Samba Virus
Method: EM (tomography)

EMDB-20747:
Low pH-Treated Samba Virus
Method: EM (tomography)

EMDB-20748:
High temperature-incubated Samba virus
Method: EM (tomography)

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