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TitleStructure and tethering mechanism of dynein-2 intermediate chains in intraflagellar transport.
Journal, issue, pagesEMBO J, Vol. 43, Issue 7, Page 1257-1272, Year 2024
Publish dateMar 7, 2024
AuthorsAakash G Mukhopadhyay / Katerina Toropova / Lydia Daly / Jennifer N Wells / Laura Vuolo / Miroslav Mladenov / Marian Seda / Dagan Jenkins / David J Stephens / Anthony J Roberts /
PubMed AbstractDynein-2 is a large multiprotein complex that powers retrograde intraflagellar transport (IFT) of cargoes within cilia/flagella, but the molecular mechanism underlying this function is still emerging. ...Dynein-2 is a large multiprotein complex that powers retrograde intraflagellar transport (IFT) of cargoes within cilia/flagella, but the molecular mechanism underlying this function is still emerging. Distinctively, dynein-2 contains two identical force-generating heavy chains that interact with two different intermediate chains (WDR34 and WDR60). Here, we dissect regulation of dynein-2 function by WDR34 and WDR60 using an integrative approach including cryo-electron microscopy and CRISPR/Cas9-enabled cell biology. A 3.9 Å resolution structure shows how WDR34 and WDR60 use surprisingly different interactions to engage equivalent sites of the two heavy chains. We show that cilia can assemble in the absence of either WDR34 or WDR60 individually, but not both subunits. Dynein-2-dependent distribution of cargoes depends more strongly on WDR60, because the unique N-terminal extension of WDR60 facilitates dynein-2 targeting to cilia. Strikingly, this N-terminal extension can be transplanted onto WDR34 and retain function, suggesting it acts as a flexible tether to the IFT "trains" that assemble at the ciliary base. We discuss how use of unstructured tethers represents an emerging theme in IFT train interactions.
External linksEMBO J / PubMed:38454149 / PubMed Central
MethodsEM (single particle) / X-ray diffraction
Resolution2.02 - 4.0 Å
Structure data

19132

8rgg

EMDB-19132, PDB-8rgg:
Structure of dynein-2 intermediate chain DYNC2I2 (WDR34) in complex with dynein-2 heavy chain DYNC2H1.
Method: EM (single particle) / Resolution: 4.0 Å

19133

8rgh

EMDB-19133, PDB-8rgh:
Structure of dynein-2 intermediate chain DYNC2I1 (WDR60) in complex with the dynein-2 heavy chain DYNC2H1.
Method: EM (single particle) / Resolution: 3.9 Å

PDB-8rgi:
Structure of DYNLT1:DYNLT2B (TCTEX1:TCTEX1D2) heterodimer.
Method: X-RAY DIFFRACTION / Resolution: 2.02 Å

Source
  • homo sapiens (human)
KeywordsTRANSPORT PROTEIN / dynein / cilia / intraflagellar transport / complex

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