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TitleStructure and mechanisms of transport of human Asc1/CD98hc amino acid transporter.
Journal, issue, pagesNat Commun, Vol. 15, Issue 1, Page 2986, Year 2024
Publish dateApr 6, 2024
AuthorsJosep Rullo-Tubau / Maria Martinez-Molledo / Paola Bartoccioni / Ignasi Puch-Giner / Ángela Arias / Suwipa Saen-Oon / Camille Stephan-Otto Attolini / Rafael Artuch / Lucía Díaz / Víctor Guallar / Ekaitz Errasti-Murugarren / Manuel Palacín / Oscar Llorca /
PubMed AbstractRecent cryoEM studies elucidated details of the structural basis for the substrate selectivity and translocation of heteromeric amino acid transporters. However, Asc1/CD98hc is the only neutral ...Recent cryoEM studies elucidated details of the structural basis for the substrate selectivity and translocation of heteromeric amino acid transporters. However, Asc1/CD98hc is the only neutral heteromeric amino acid transporter that can function through facilitated diffusion, and the only one that efficiently transports glycine and D-serine, and thus has a regulatory role in the central nervous system. Here we use cryoEM, ligand-binding simulations, mutagenesis, transport assays, and molecular dynamics to define human Asc1/CD98hc determinants for substrate specificity and gain insights into the mechanisms that govern substrate translocation by exchange and facilitated diffusion. The cryoEM structure of Asc1/CD98hc is determined at 3.4-3.8 Å resolution, revealing an inward-facing semi-occluded conformation. We find that Ser 246 and Tyr 333 are essential for Asc1/CD98hc substrate selectivity and for the exchange and facilitated diffusion modes of transport. Taken together, these results reveal the structural bases for ligand binding and transport features specific to human Asc1.
External linksNat Commun / PubMed:38582862 / PubMed Central
MethodsEM (single particle)
Resolution4.0 Å
Structure data

EMDB-18379, PDB-8qey:
Structure of human Asc1/CD98hc heteromeric amino acid transporter
Method: EM (single particle) / Resolution: 4.0 Å

Chemicals

ChemComp-AJP:
Digitonin / detergent*YM / Digitonin

ChemComp-NAG:
2-acetamido-2-deoxy-beta-D-glucopyranose / N-Acetylglucosamine

Source
  • homo sapiens (human)
KeywordsMEMBRANE PROTEIN / Heteromeric amino acid transporter Transporter APC transporter

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