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TitleUnconventional stabilization of the human T-cell leukemia virus type 1 immature Gag lattice.
Journal, issue, pagesbioRxiv, Year 2023
Publish dateJul 24, 2023
AuthorsMartin Obr / Mathias Percipalle / Darya Chernikova / Huixin Yang / Andreas Thader / Gergely Pinke / Dario Porley / Louis M Mansky / Robert A Dick / Florian Km Schur /
PubMed AbstractHuman T-cell leukemia virus type 1 (HTLV-1) has an atypical immature particle morphology compared to other retroviruses. This indicates that these particles are formed in a way that is unique. Here ...Human T-cell leukemia virus type 1 (HTLV-1) has an atypical immature particle morphology compared to other retroviruses. This indicates that these particles are formed in a way that is unique. Here we report the results of cryo-electron tomography (cryo-ET) studies of HTLV-1 virus-like particles (VLPs) assembled , as well as derived from cells. This work shows that HTLV-1 employs an unconventional mechanism of Gag-Gag interactions to form the immature viral lattice. Analysis of high-resolution structural information from immature CA tubular arrays reveals that the primary stabilizing component in HTLV-1 is CA-NTD. Mutagenesis and biophysical analysis support this observation. This distinguishes HTLV-1 from other retroviruses, in which the stabilization is provided primarily by the CA-CTD. These results are the first to provide structural details of the quaternary arrangement of Gag for an immature deltaretrovirus, and this helps explain why HTLV-1 particles are morphologically distinct.
External linksbioRxiv / PubMed:37546793 / PubMed Central
MethodsEM (subtomogram averaging) / EM (tomography)
Resolution3.4 - 6.9 Å
Structure data

17929

8pu6

EMDB-17929, PDB-8pu6:
Structure of immature HTLV-1 CA-NTD from in vitro assembled MA126-CANC tubes: pooled class
Method: EM (subtomogram averaging) / Resolution: 3.4 Å

17930

8pu7

EMDB-17930, PDB-8pu7:
Structure of immature HTLV-1 CA-NTD from in vitro assembled MA126-CANC tubes: axis angle -20 degrees
Method: EM (subtomogram averaging) / Resolution: 4.8 Å

17931

8pu8

EMDB-17931, PDB-8pu8:
Structure of immature HTLV-1 CA-NTD from in vitro assembled MA126-CANC tubes: axis angle -15 degrees
Method: EM (subtomogram averaging) / Resolution: 4.0 Å

17932

8pu9

EMDB-17932, PDB-8pu9:
Structure of immature HTLV-1 CA-NTD from in vitro assembled MA126-CANC tubes: axis angle -10 degrees
Method: EM (subtomogram averaging) / Resolution: 3.7 Å

17933

8pua

EMDB-17933, PDB-8pua:
Structure of immature HTLV-1 CA-NTD from in vitro assembled MA126-CANC tubes: axis angle -05 degrees
Method: EM (subtomogram averaging) / Resolution: 3.7 Å

17934

8pub

EMDB-17934, PDB-8pub:
Structure of immature HTLV-1 CA-NTD from in vitro assembled MA126-CANC tubes: axis angle 0 degrees
Method: EM (subtomogram averaging) / Resolution: 4.0 Å

17935

8puc

EMDB-17935, PDB-8puc:
Structure of immature HTLV-1 CA-NTD from in vitro assembled MA126-CANC tubes: axis angle 05 degrees
Method: EM (subtomogram averaging) / Resolution: 4.3 Å

17936

8pud

EMDB-17936, PDB-8pud:
Structure of immature HTLV-1 CA-NTD from in vitro assembled MA126-CANC tubes: axis angle 10 degrees
Method: EM (subtomogram averaging) / Resolution: 4.5 Å

17937

8pue

EMDB-17937, PDB-8pue:
Structure of immature HTLV-1 CA-NTD from in vitro assembled MA126-CANC tubes: axis angle 15 degrees
Method: EM (subtomogram averaging) / Resolution: 6.9 Å

EMDB-17938: Cryo-electron tomogram of HTLV-1 MA126CANC tubes
Method: EM (tomography)

EMDB-17939: Cryo-electron tomogram of HTLV-1 MA126CANC tubes
Method: EM (tomography)

17940

8puf

EMDB-17940, PDB-8puf:
Structure of immature HTLV-1 CA-NTD from in vitro assembled MA126-CANC tubes: axis angle 20 degrees
Method: EM (subtomogram averaging) / Resolution: 6.1 Å

EMDB-17941: Cryo-electron tomogram containing HTLV-1 Gag-based VLPs
Method: EM (tomography)

17942

8pug

EMDB-17942, PDB-8pug:
Structure of the immature HTLV-1 CA lattice from full-length Gag VLPs: CA-NTD refinement
Method: EM (subtomogram averaging) / Resolution: 5.9 Å

17943

8puh

EMDB-17943, PDB-8puh:
Structure of the immature HTLV-1 CA lattice from full-length Gag VLPs: CA-CTD refinement
Method: EM (subtomogram averaging) / Resolution: 6.2 Å

Source
  • human t-cell leukemia virus type i
KeywordsVIRAL PROTEIN / Retrovirus / HTLV / immature capsid / CA / CA-NTD

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