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Title | Structure of hibernating ribosomes studied by cryoelectron tomography in vitro and in situ. |
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Journal, issue, pages | J Cell Biol, Vol. 190, Issue 4, Page 613-621, Year 2010 |
Publish date | Aug 23, 2010 |
Authors | Julio O Ortiz / Florian Brandt / Valério R F Matias / Lau Sennels / Juri Rappsilber / Sjors H W Scheres / Matthias Eibauer / F Ulrich Hartl / Wolfgang Baumeister / |
PubMed Abstract | Ribosomes arranged in pairs (100S) have been related with nutritional stress response and are believed to represent a "hibernation state." Several proteins have been identified that are associated ...Ribosomes arranged in pairs (100S) have been related with nutritional stress response and are believed to represent a "hibernation state." Several proteins have been identified that are associated with 100S ribosomes but their spatial organization has hitherto not been characterized. We have used cryoelectron tomography to reveal the three-dimensional configuration of 100S ribosomes isolated from starved Escherichia coli cells and we have described their mode of interaction. In situ studies with intact E. coli cells allowed us to demonstrate that 100S ribosomes do exist in vivo and represent an easily reversible state of quiescence; they readily vanish when the growth medium is replenished. |
External links | J Cell Biol / PubMed:20733057 / PubMed Central |
Methods | EM (subtomogram averaging) |
Structure data | EMDB-1750: |
Source |
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