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TitleStructure of the transcribing RNA polymerase II-Elongin complex.
Journal, issue, pagesNat Struct Mol Biol, Vol. 30, Issue 12, Page 1925-1935, Year 2023
Publish dateNov 6, 2023
AuthorsYing Chen / Goran Kokic / Christian Dienemann / Olexandr Dybkov / Henning Urlaub / Patrick Cramer /
PubMed AbstractElongin is a heterotrimeric elongation factor for RNA polymerase (Pol) II transcription that is conserved among metazoa. Here, we report three cryo-EM structures of human Elongin bound to ...Elongin is a heterotrimeric elongation factor for RNA polymerase (Pol) II transcription that is conserved among metazoa. Here, we report three cryo-EM structures of human Elongin bound to transcribing Pol II. The structures show that Elongin subunit ELOA binds the RPB2 side of Pol II and anchors the ELOB-ELOC subunit heterodimer. ELOA contains a 'latch' that binds between the end of the Pol II bridge helix and funnel helices, thereby inducing a conformational change near the polymerase active center. The latch is required for the elongation-stimulatory activity of Elongin, but not for Pol II binding, indicating that Elongin functions by allosterically regulating the conformational mobility of the polymerase active center. Elongin binding to Pol II is incompatible with association of the super elongation complex, PAF1 complex and RTF1, which also contain an elongation-stimulatory latch element.
External linksNat Struct Mol Biol / PubMed:37932450 / PubMed Central
MethodsEM (single particle)
Resolution2.69 - 4.2 Å
Structure data

EMDB-16828: Structure of the mammalian Pol II-SPT6 complex, Struture 4 (Global map)
Method: EM (single particle) / Resolution: 3.04 Å

EMDB-16829: Structure of the mammalian Pol II-SPT6 complex, Structure 4 (Focused map for SPT6)
Method: EM (single particle) / Resolution: 3.64 Å

EMDB-16830: Structure of the mammalian Pol II-Elongin complex, lacking ELOA latch (Focused map for elongin, map 5)
Method: EM (single particle) / Resolution: 3.61 Å

EMDB-16831: Structure of the mammalian Pol II-Elongin complex, lacking ELOA latch (Focused map for Pol II core, Map 6)
Method: EM (single particle) / Resolution: 2.69 Å

EMDB-16832: Structure of the mammalian Pol II-Elongin complex, lacking ELOA latch (Focused map for Pol II stalk, map 7)
Method: EM (single particle) / Resolution: 3.67 Å

EMDB-16833: Structure of the mammalian Pol II-SPT6-Elongin complex, lacking the ELOA latch (global map for composite map 2)
Method: EM (single particle) / Resolution: 2.86 Å

EMDB-16834: Structure of the mammalian Pol II-SPT6-Elongin complex, lacking ELOA latch (SPT6 focused map for composite map 2)
Method: EM (single particle) / Resolution: 4.2 Å

16835

8oeu

EMDB-16835: Structure of the mammalian Pol II-SPT6 complex (composite map)
PDB-8oeu: Structure of the mammalian Pol II-SPT6 complex (composite structure, Structure 4)
Method: EM (single particle) / Resolution: 3.04 Å

EMDB-16836: Structure of the Pol II-SPT6-Elongin complex (structure 1, global map)
Method: EM (single particle) / Resolution: 3.05 Å

16837

8oev

EMDB-16837: Structure of the mammalian Pol II-SPT6-Elongin complex, lacking ELOA latch (composite map 2)
PDB-8oev: Structure of the mammalian Pol II-SPT6-Elongin complex, lacking ELOA latch (composite structure, structure 3)
Method: EM (single particle) / Resolution: 2.86 Å

16838

8oew

EMDB-16838: Structure of the mammalian Pol II-Elongin complex, lacking the ELOA latch (composite map 1 for structure 2)
PDB-8oew: Structure of the mammalian Pol II-Elongin complex, lacking the ELOA latch (composite structure, structure 2)
Method: EM (single particle) / Resolution: 2.8 Å

EMDB-16839: Structure of the mammalian Pol II-Elongin complex, lacking the ELOA latch (local resolution filtered map)
Method: EM (single particle) / Resolution: 3.67 Å

16840

8of0

EMDB-16840: Structure of the mammalian Pol II-SPT6-Elongin complex, Structure 1 (local resolution filtered map)
PDB-8of0: Structure of the mammalian Pol II-SPT6-Elongin complex, Structure 1
Method: EM (single particle) / Resolution: 3.05 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-ZN:
Unknown entry

Source
  • homo sapiens (human)
  • sus scrofa (pig)
  • synthetic construct (others)
KeywordsTRANSCRIPTION / Transcription elongation / Elongin / RNA polymerase II

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