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TitleThe architecture of Cidec-mediated interfaces between lipid droplets.
Journal, issue, pagesCell Rep, Vol. 42, Issue 2, Page 112107, Year 2023
Publish dateFeb 28, 2023
AuthorsIva Ganeva / Koini Lim / Jerome Boulanger / Patrick C Hoffmann / Olivia Muriel / Alicia C Borgeaud / Wim J H Hagen / David B Savage / Wanda Kukulski /
PubMed AbstractLipid droplets (LDs) are intracellular organelles responsible for storing surplus energy as neutral lipids. Their size and number vary enormously. In white adipocytes, LDs can reach 100 μm in ...Lipid droplets (LDs) are intracellular organelles responsible for storing surplus energy as neutral lipids. Their size and number vary enormously. In white adipocytes, LDs can reach 100 μm in diameter, occupying >90% of the cell. Cidec, which is strictly required for the formation of large LDs, is concentrated at interfaces between adjacent LDs and facilitates directional flux of neutral lipids from the smaller to the larger LD. The mechanism of lipid transfer is unclear, in part because the architecture of interfaces between LDs remains elusive. Here we visualize interfaces between LDs by electron cryo-tomography and analyze the kinetics of lipid transfer by quantitative live fluorescence microscopy. We show that transfer occurs through closely apposed monolayers, is slowed down by increasing the distance between the monolayers, and follows exponential kinetics. Our data corroborate the notion that Cidec facilitates pressure-driven transfer of neutral lipids through two "leaky" monolayers between LDs.
External linksCell Rep / PubMed:36800289 / PubMed Central
MethodsEM (tomography)
Structure data

EMDB-16454: Electron cryo-tomography of HeLa cells expressing untagged Cidec
Method: EM (tomography)

EMDB-16455: Electron cryo-tomography of HeLa cells expressing Cidec-EGFP
Method: EM (tomography)

Source
  • Homo sapiens (human)

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