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-Structure paper
Title | Morphological Diversity of Dps Complex with Genomic DNA. |
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Journal, issue, pages | Int J Mol Sci, Vol. 24, Issue 10, Year 2023 |
Publish date | May 10, 2023 |
Authors | Yuri Chesnokov / Roman Kamyshinsky / Andrey Mozhaev / Eleonora Shtykova / Alexander Vasiliev / Ivan Orlov / Liubov Dadinova / |
PubMed Abstract | In response to adverse environmental factors, cells actively produce Dps proteins which form ordered complexes (biocrystals) with bacterial DNA to protect the genome. The effect of ...In response to adverse environmental factors, cells actively produce Dps proteins which form ordered complexes (biocrystals) with bacterial DNA to protect the genome. The effect of biocrystallization has been described extensively in the scientific literature; furthermore, to date, the structure of the Dps-DNA complex has been established in detail in vitro using plasmid DNA. In the present work, for the first time, Dps complexes with genomic DNA were studied in vitro using cryo-electron tomography. We demonstrate that genomic DNA forms one-dimensional crystals or filament-like assemblies which transform into weakly ordered complexes with triclinic unit cells, similar to what is observed for plasmid DNA. Changing such environmental factors as pH and KCl and MgCl concentrations leads to the formation of cylindrical structures. |
External links | Int J Mol Sci / PubMed:37239879 / PubMed Central |
Methods | EM (subtomogram averaging) |
Resolution | 22.0 - 30.5 Å |
Structure data | EMDB-16438: Toroidal Dps-DNA assembly EMDB-16439: Dps-DNA filament-like assembly |
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