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TitleDNA-origami-directed virus capsid polymorphism.
Journal, issue, pagesNat Nanotechnol, Vol. 18, Issue 10, Page 1205-1212, Year 2023
Publish dateJul 17, 2023
AuthorsIris Seitz / Sharon Saarinen / Esa-Pekka Kumpula / Donna McNeale / Eduardo Anaya-Plaza / Vili Lampinen / Vesa P Hytönen / Frank Sainsbury / Jeroen J L M Cornelissen / Veikko Linko / Juha T Huiskonen / Mauri A Kostiainen /
PubMed AbstractViral capsids can adopt various geometries, most iconically characterized by icosahedral or helical symmetries. Importantly, precise control over the size and shape of virus capsids would have ...Viral capsids can adopt various geometries, most iconically characterized by icosahedral or helical symmetries. Importantly, precise control over the size and shape of virus capsids would have advantages in the development of new vaccines and delivery systems. However, current tools to direct the assembly process in a programmable manner are exceedingly elusive. Here we introduce a modular approach by demonstrating DNA-origami-directed polymorphism of single-protein subunit capsids. We achieve control over the capsid shape, size and topology by employing user-defined DNA origami nanostructures as binding and assembly platforms, which are efficiently encapsulated within the capsid. Furthermore, the obtained viral capsid coatings can shield the encapsulated DNA origami from degradation. Our approach is, moreover, not limited to a single type of capsomers and can also be applied to RNA-DNA origami structures to pave way for next-generation cargo protection and targeting strategies.
External linksNat Nanotechnol / PubMed:37460794 / PubMed Central
MethodsEM (single particle)
Resolution4.3 - 10.2 Å
Structure data

16076

8bi4

EMDB-16076, PDB-8bi4:
Helical shell of CCMV capsid protein on DNA origami 6HB-2k
Method: EM (single particle) / Resolution: 4.3 Å

EMDB-16077: Inner helical shell of CCMV capsid protein on DNA origami 6HB-10k
Method: EM (single particle) / Resolution: 7.3 Å

EMDB-16078: Outer helical shell of CCMV capsid protein on DNA origami 6HB-10k
Method: EM (single particle) / Resolution: 7.0 Å

EMDB-16079: Helical shell cap of CCMV capsid protein on DNA origami 6HB-2k
Method: EM (single particle) / Resolution: 8.8 Å

EMDB-16080: Helical shell of CCMV capsid protein on DNA origami 24HB-2.5k
Method: EM (single particle) / Resolution: 10.2 Å

Source
  • cowpea chlorotic mottle virus
KeywordsVIRUS LIKE PARTICLE / origami / capsid

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