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TitleElectron microscopy reveals toroidal shape of master neuronal cell differentiator REST - RE1-silencing transcription factor.
Journal, issue, pagesComput Struct Biotechnol J, Vol. 21, Page 731-741, Year 2023
Publish dateDec 19, 2022
AuthorsPavel Veverka / Tomáš Brom / Tomáš Janovič / Martin Stojaspal / Matyáš Pinkas / Jiří Nováček / Ctirad Hofr /
PubMed AbstractThe RE1-Silencing Transcription factor (REST) is essential for neuronal differentiation. Here, we report the first 18.5-angstrom electron microscopy structure of human REST. The refined electron map ...The RE1-Silencing Transcription factor (REST) is essential for neuronal differentiation. Here, we report the first 18.5-angstrom electron microscopy structure of human REST. The refined electron map suggests that REST forms a torus that can accommodate DNA double-helix in the central hole. Additionally, we quantitatively described REST binding to the canonical DNA sequence of the neuron-restrictive silencer element. We developed protocols for the expression and purification of full-length REST and the shortened variant REST-N62 produced by alternative splicing. We tested the mutual interaction of full-length REST and the splicing variant REST-N62. Revealed structure-function relationships of master neuronal repressor REST will allow finding new biological ways of prevention and treatment of neurodegenerative disorders and diseases.
External linksComput Struct Biotechnol J / PubMed:36698979 / PubMed Central
MethodsEM (single particle)
Resolution18.5 Å
Structure data

EMDB-15858: Negative stain EM map of human RE1-silencing transcription factor
Method: EM (single particle) / Resolution: 18.5 Å

Source
  • Homo sapiens (human)

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