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TitleStructure shows that the BIR2 domain of E3 ligase XIAP binds across the RIPK2 kinase dimer interface.
Journal, issue, pagesLife Sci Alliance, Vol. 6, Issue 11, Year 2023
Publish dateSep 6, 2023
AuthorsMathilde Lethier / Karine Huard / Michael Hons / Adrien Favier / Bernhard Brutscher / Elisabetta Boeri Erba / Derek W Abbott / Stephen Cusack / Erika Pellegrini /
PubMed AbstractRIPK2 is an essential adaptor for NOD signalling and its kinase domain is a drug target for NOD-related diseases, such as inflammatory bowel disease. However, recent work indicates that the ...RIPK2 is an essential adaptor for NOD signalling and its kinase domain is a drug target for NOD-related diseases, such as inflammatory bowel disease. However, recent work indicates that the phosphorylation activity of RIPK2 is dispensable for signalling and that inhibitors of both RIPK2 activity and RIPK2 ubiquitination prevent the essential interaction between RIPK2 and the BIR2 domain of XIAP, the key RIPK2 ubiquitin E3 ligase. Moreover, XIAP BIR2 antagonists also block this interaction. To reveal the molecular mechanisms involved, we combined native mass spectrometry, NMR, and cryo-electron microscopy to determine the structure of the RIPK2 kinase BIR2 domain complex and validated the interface with in cellulo assays. The structure shows that BIR2 binds across the RIPK2 kinase antiparallel dimer and provides an explanation for both inhibitory mechanisms. It also highlights why phosphorylation of the kinase activation loop is dispensable for signalling while revealing the structural role of RIPK2-K209 residue in the RIPK2-XIAP BIR2 interaction. Our results clarify the features of the RIPK2 conformation essential for its role as a scaffold protein for ubiquitination.
External linksLife Sci Alliance / PubMed:37673444 / PubMed Central
MethodsEM (single particle)
Resolution3.15 Å
Structure data

15757

8aza

EMDB-15757, PDB-8aza:
Structure of RIP2K dimer bound to the XIAP BIR2 domain
Method: EM (single particle) / Resolution: 3.15 Å

Chemicals

ChemComp-ZN:
Unknown entry

Source
  • homo sapiens (human)
KeywordsIMMUNE SYSTEM / kinase / BIR2 / complex / dimer

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