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TitleCryo-EM structures of perforin-2 in isolation and assembled on a membrane suggest a mechanism for pore formation.
Journal, issue, pagesEMBO J, Vol. 41, Issue 23, Page e111857, Year 2022
Publish dateDec 1, 2022
AuthorsXiulian Yu / Tao Ni / George Munson / Peijun Zhang / Robert J C Gilbert /
PubMed AbstractPerforin-2 (PFN2, MPEG1) is a key pore-forming protein in mammalian innate immunity restricting intracellular bacteria proliferation. It forms a membrane-bound pre-pore complex that converts to a ...Perforin-2 (PFN2, MPEG1) is a key pore-forming protein in mammalian innate immunity restricting intracellular bacteria proliferation. It forms a membrane-bound pre-pore complex that converts to a pore-forming structure upon acidification; but its mechanism of conformational transition has been debated. Here we used cryo-electron microscopy, tomography and subtomogram averaging to determine structures of PFN2 in pre-pore and pore conformations in isolation and bound to liposomes. In isolation and upon acidification, the pre-assembled complete pre-pore rings convert to pores in both flat ring and twisted conformations. On membranes, in situ assembled PFN2 pre-pores display various degrees of completeness; whereas PFN2 pores are mainly incomplete arc structures that follow the same subunit packing arrangements as found in isolation. Both assemblies on membranes use their P2 β-hairpin for binding to the lipid membrane surface. Overall, these structural snapshots suggest a molecular mechanism for PFN2 pre-pore to pore transition on a targeted membrane, potentially using the twisted pore as an intermediate or alternative state to the flat conformation, with the capacity to cause bilayer distortion during membrane insertion.
External linksEMBO J / PubMed:36245269 / PubMed Central
MethodsEM (single particle) / EM (subtomogram averaging)
Resolution3.0 - 18.0 Å
Structure data

15072

8a1d

EMDB-15072, PDB-8a1d:
Structure of murine perforin-2 (Mpeg1) pore in ring form
Method: EM (single particle) / Resolution: 3.0 Å

EMDB-15076: murine perforin-2 (Mpeg1) prepore on membrane by cryoET subtomogram averaging
Method: EM (subtomogram averaging) / Resolution: 6.0 Å

15086

8a1s

EMDB-15086, PDB-8a1s:
Structure of murine perforin-2 (Mpeg1) pore in twisted form
Method: EM (single particle) / Resolution: 4.0 Å

EMDB-15087: murine perforin-2 pore on liposome by subtomogram averaging
Method: EM (subtomogram averaging) / Resolution: 18.0 Å

Chemicals

ChemComp-NAG:
2-acetamido-2-deoxy-beta-D-glucopyranose / N-Acetylglucosamine

ChemComp-MA4:
CYCLOHEXYL-HEXYL-BETA-D-MALTOSIDE

Source
  • mus musculus (house mouse)
KeywordsIMMUNE SYSTEM / pore forming protein / MACPF / beta-barrel / immunity

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