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| Title | The role of the kinesin-13 neck in microtubule depolymerization. |
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| Journal, issue, pages | Cell Cycle, Vol. 5, Issue 16, Page 1812-1815, Year 2006 |
| Publish date | Aug 15, 2006 |
 Authors | Carolyn A Moores / Jeremy Cooper / Mike Wagenbach / Yulia Ovechkina / Linda Wordeman / Ronald A Milligan /  |
| PubMed Abstract | To ensure genetic integrity, replicated chromosomes must be accurately distributed to daughter cells-a process that is accomplished on the microtubule spindle. Kinesin-13 motors play an essential ...To ensure genetic integrity, replicated chromosomes must be accurately distributed to daughter cells-a process that is accomplished on the microtubule spindle. Kinesin-13 motors play an essential role in this process by performing regulated microtubule depolymerization. We set out to dissect the depolymerization mechanism of these kinesins, and in particular, the role of their conserved neck sequence. We used a monomeric kinesin-13 MCAK, consisting of the neck and motor core, which has strong depolymerizing activity. In the presence of a non-hydrolysable ATP analogue, this construct induced formation of rings around microtubules. The rings are built from tubulin protofilaments that are bent by the kinesin-13 motor engaged at the ATP-binding step of its ATPase cycle. Our data suggest that the ring-microtubule interaction is mediated by the neck and support the idea of a role for the kinesin-13 neck in depolymerization efficiency, acting by optimizing release of tubulin from microtubule ends. |
 External links | Cell Cycle / PubMed:16929184 |
| Methods | EM (helical sym.) |
| Resolution | 20.0 Å |
| Structure data |  EMDB-1441:  EMDB-1442:  EMDB-1443:  EMDB-1444: |
| Source |
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Plasmodium falciparum (malaria parasite P. falciparum)