Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Modular assembly of the principal microtubule nucleator γ-TuRC.
Journal, issue, pages	Nat Commun, Vol. 13, Issue 1, Page 473, Year 2022
Publish date	Jan 25, 2022
Authors	Martin Würtz / Erik Zupa / Enrico S Atorino / Annett Neuner / Anna Böhler / Ariani S Rahadian / Bram J A Vermeulen / Giulia Tonon / Sebastian Eustermann / Elmar Schiebel / Stefan Pfeffer /
PubMed Abstract	The gamma-tubulin ring complex (γ-TuRC) is the principal microtubule nucleation template in vertebrates. Recent cryo-EM reconstructions visualized the intricate quaternary structure of the γ-TuRC, ...The gamma-tubulin ring complex (γ-TuRC) is the principal microtubule nucleation template in vertebrates. Recent cryo-EM reconstructions visualized the intricate quaternary structure of the γ-TuRC, containing more than thirty subunits, raising fundamental questions about γ-TuRC assembly and the role of actin as an integral part of the complex. Here, we reveal the structural mechanism underlying modular γ-TuRC assembly and identify a functional role of actin in microtubule nucleation. During γ-TuRC assembly, a GCP6-stabilized core comprising GCP2-3-4-5-4-6 is expanded by stepwise recruitment, selective stabilization and conformational locking of four pre-formed GCP2-GCP3 units. Formation of the lumenal bridge specifies incorporation of the terminal GCP2-GCP3 unit and thereby leads to closure of the γ-TuRC ring in a left-handed spiral configuration. Actin incorporation into the complex is not relevant for γ-TuRC assembly and structural integrity, but determines γ-TuRC geometry and is required for efficient microtubule nucleation and mitotic chromosome alignment in vivo.
External links	Nat Commun / PubMed:35078983 / PubMed Central
Methods	EM (single particle)
Resolution	5.32 - 16.1 Å
Structure data	14005 7qj0 EMDB-14005, PDB-7qj0: Structure of recombinant human gamma-Tubulin Ring Complex 6-spoked assembly intermediate (spokes 7-12) Method: EM (single particle) / Resolution: 5.32 Å 14006 7qj1 EMDB-14006, PDB-7qj1: Structure of the recombinant human gamma-Tubulin Ring Complex 6-spoked assembly intermediate (spokes 7-12, homogeneous dataset) Method: EM (single particle) / Resolution: 7.0 Å 14007 7qj2 EMDB-14007, PDB-7qj2: Structure of recombinant human gamma-Tubulin Ring Complex 8-spoked assembly intermediate (spokes 5-12) Method: EM (single particle) / Resolution: 8.6 Å 14008 7qj3 EMDB-14008, PDB-7qj3: Structure of recombinant human gamma-Tubulin Ring Complex 8-spoked assembly intermediate (spokes 7-14) Method: EM (single particle) / Resolution: 7.6 Å 14009 7qj4 EMDB-14009, PDB-7qj4: Structure of recombinant human gamma-Tubulin Ring Complex 10-spoked assembly intermediate (spokes 5-14) Method: EM (single particle) / Resolution: 9.0 Å 14010 7qj5 EMDB-14010, PDB-7qj5: Structure of recombinant human gamma-Tubulin Ring Complex (spokes 1-14) Method: EM (single particle) / Resolution: 8.7 Å 14011 7qj6 EMDB-14011, PDB-7qj6: Structure of recombinant human gamma-Tubulin Ring Complex 10-spoked assembly intermediate (spokes 3-12, substoichiometric spokes 13-14) Method: EM (single particle) / Resolution: 7.8 Å 14012 7qj7 EMDB-14012, PDB-7qj7: Structure of recombinant human gamma-Tubulin Ring Complex 12-spoked assembly intermediate (spokes 1-12 substoichiometric spokes 13-14) Method: EM (single particle) / Resolution: 8.7 Å 14013 7qj8 EMDB-14013, PDB-7qj8: Structure of recombinant human gamma-Tubulin Ring Complex 12-spoked assembly intermediate (spokes 3-14, substoichiometric spokes 1-2) Method: EM (single particle) / Resolution: 8.7 Å 14014 7qj9 EMDB-14014, PDB-7qj9: Structure of recombinant human gamma-Tubulin Ring Complex 10-spoked assembly intermediate (spokes 3-12, homogeneous dataset) Method: EM (single particle) / Resolution: 8.1 Å 14015 7qja EMDB-14015, PDB-7qja: Structure of recombinant human gamma-Tubulin Ring Complex 12-spoked assembly intermediate (spokes 1-12, homogeneous dataset) Method: EM (single particle) / Resolution: 9.2 Å 14016 7qjb EMDB-14016, PDB-7qjb: Structure of recombinant human gamma-Tubulin Ring Complex 12-spoked assembly intermediate (spokes 3-14, homogeneous dataset) Method: EM (single particle) / Resolution: 9.2 Å 14017 7qjc EMDB-14017, PDB-7qjc: Structure of recombinant human gamma-Tubulin Ring Complex (spokes 1-14, homogeneous dataset) Method: EM (single particle) / Resolution: 16.1 Å 14018 7qjd EMDB-14018, PDB-7qjd: Structure of recombinant human gamma-Tubulin Ring Complex without actin Method: EM (single particle) / Resolution: 7.1 Å 14019 7qje EMDB-14019, PDB-7qje: Structure of recombinant human gamma-Tubulin Ring Complex 4-spoked assembly intermediate (spokes 9-12) Method: EM (single particle) / Resolution: 7.8 Å
Chemicals	ChemComp-HOH: WATER / Water
Source	homo sapiens (human)
Keywords	CYTOSOLIC PROTEIN / Assembly / Intermediate / Complex