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TitleMolecular mechanism of SbmA, a promiscuous transporter exploited by antimicrobial peptides.
Journal, issue, pagesSci Adv, Vol. 7, Issue 37, Page eabj5363, Year 2021
Publish dateSep 10, 2021
AuthorsDmitry Ghilarov / Satomi Inaba-Inoue / Piotr Stepien / Feng Qu / Elizabeth Michalczyk / Zuzanna Pakosz / Norimichi Nomura / Satoshi Ogasawara / Graham Charles Walker / Sylvie Rebuffat / So Iwata / Jonathan Gardiner Heddle / Konstantinos Beis /
PubMed AbstractAntibiotic metabolites and antimicrobial peptides mediate competition between bacterial species. Many of them hijack inner and outer membrane proteins to enter cells. Sensitivity of enteric bacteria ...Antibiotic metabolites and antimicrobial peptides mediate competition between bacterial species. Many of them hijack inner and outer membrane proteins to enter cells. Sensitivity of enteric bacteria to multiple peptide antibiotics is controlled by the single inner membrane protein SbmA. To establish the molecular mechanism of peptide transport by SbmA and related BacA, we determined their cryo–electron microscopy structures at 3.2 and 6 Å local resolution, respectively. The structures show a previously unknown fold, defining a new class of secondary transporters named SbmA-like peptide transporters. The core domain includes conserved glutamates, which provide a pathway for proton translocation, powering transport. The structures show an outward-open conformation with a large cavity that can accommodate diverse substrates. We propose a molecular mechanism for antibacterial peptide uptake paving the way for creation of narrow-targeted therapeutics.
External linksSci Adv / PubMed:34516884 / PubMed Central
MethodsEM (single particle)
Resolution3.59 - 6.36 Å
Structure data

13168

7p34

EMDB-13168: SbmA-FabS11-1 in lipid nanodisc
PDB-7p34: Cryo-EM structure of the proton-dependent antibacterial peptide transporter SbmA-FabS11-1 in nanodiscs
Method: EM (single particle) / Resolution: 3.59 Å

EMDB-13173:
Proton-powered peptide transporter SbmA in lipid nanodisc
Method: EM (single particle) / Resolution: 3.9 Å

EMDB-13175:
Peptide transporter BacA in lipid nanodisc
Method: EM (single particle) / Resolution: 6.36 Å

Chemicals

ChemComp-PGW:
(1R)-2-{[(S)-{[(2S)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl / phospholipid*YM / Phosphatidylglycerol

Source
  • escherichia coli (E. coli)
  • Sinorhizobium meliloti (bacteria)
KeywordsTRANSPORT PROTEIN / SLiPT / proton transport / peptide transport / antibiotics

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