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TitleMulti-modal adaptor-clathrin contacts drive coated vesicle assembly.
Journal, issue, pagesEMBO J, Vol. 40, Issue 19, Page e108795, Year 2021
Publish dateOct 1, 2021
AuthorsSarah M Smith / Gabrielle Larocque / Katherine M Wood / Kyle L Morris / Alan M Roseman / Richard B Sessions / Stephen J Royle / Corinne J Smith /
PubMed AbstractClathrin-coated pits are formed by the recognition of membrane and cargo by the AP2 complex and the subsequent recruitment of clathrin triskelia. A role for AP2 in coated-pit assembly beyond initial ...Clathrin-coated pits are formed by the recognition of membrane and cargo by the AP2 complex and the subsequent recruitment of clathrin triskelia. A role for AP2 in coated-pit assembly beyond initial clathrin recruitment has not been explored. Clathrin binds the β2 subunit of AP2, and several binding sites have been identified, but our structural knowledge of these interactions is incomplete and their functional importance during endocytosis is unclear. Here, we analysed the cryo-EM structure of clathrin cages assembled in the presence of β2 hinge-appendage (β2HA). We find that the β2-appendage binds in at least two positions in the cage, demonstrating that multi-modal binding is a fundamental property of clathrin-AP2 interactions. In one position, β2-appendage cross-links two adjacent terminal domains from different triskelia. Functional analysis of β2HA-clathrin interactions reveals that endocytosis requires two clathrin interaction sites: a clathrin-box motif on the hinge and the "sandwich site" on the appendage. We propose that β2-appendage binding to more than one triskelion is a key feature of the system and likely explains why assembly is driven by AP2.
External linksEMBO J / PubMed:34487371 / PubMed Central
MethodsEM (single particle)
Resolution9.1 - 15.0 Å
Structure data

EMDB-12980:
Cryo-EM map of the hexagonal face of the 28 triskelia mini clathrin coat complex- class 18
Method: EM (single particle)

EMDB-12981:
Cryo-EM structure of the 28 triskelia mini clathrin coat complex, class 18.
Method: EM (single particle) / Resolution: 15.0 Å

EMDB-12983:
Cryo-EM structure of the 28 triskelia mini clathrin coat complex
Method: EM (single particle) / Resolution: 9.1 Å

12984

7om8

EMDB-12984: Cryo-EM structure of the hub of the 28 triskelia mini clathrin coat complex, class 15
PDB-7om8: Beta2 appendage domain of AP2 bound to terminal domains beneath the hub of the 28 triskelia mini clathrin coat complex, class 15
Method: EM (single particle) / Resolution: 10.5 Å

Source
  • sus scrofa (pig)
  • homo sapiens (human)
  • pig (pig)
KeywordsENDOCYTOSIS / Clathrin / clathrin adaptor / AP2

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