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TitleStructural heterogeneity of cellular K5/K14 filaments as revealed by cryo-electron microscopy.
Journal, issue, pagesElife, Vol. 10, Year 2021
Publish dateJul 29, 2021
AuthorsMiriam S Weber / Matthias Eibauer / Suganya Sivagurunathan / Thomas M Magin / Robert D Goldman / Ohad Medalia /
PubMed AbstractKeratin intermediate filaments are an essential and major component of the cytoskeleton in epithelial cells. They form a stable yet dynamic filamentous network extending from the nucleus to the cell ...Keratin intermediate filaments are an essential and major component of the cytoskeleton in epithelial cells. They form a stable yet dynamic filamentous network extending from the nucleus to the cell periphery, which provides resistance to mechanical stresses. Mutations in keratin genes are related to a variety of epithelial tissue diseases. Despite their importance, the molecular structure of keratin filaments remains largely unknown. In this study, we analyzed the structure of keratin 5/keratin 14 filaments within ghost mouse keratinocytes by cryo-electron microscopy and cryo-electron tomography. By averaging a large number of keratin segments, we have gained insights into the helical architecture of the filaments. Two-dimensional classification revealed profound variations in the diameter of keratin filaments and their subunit organization. Computational reconstitution of filaments of substantial length uncovered a high degree of internal heterogeneity along single filaments, which can contain regions of helical symmetry, regions with less symmetry and regions with significant diameter fluctuations. Cross-section views of filaments revealed that keratins form hollow cylinders consisting of multiple protofilaments, with an electron dense core located in the center of the filament. These findings shed light on the complex and remarkable heterogenic architecture of keratin filaments, suggesting that they are highly flexible, dynamic cytoskeletal structures.
External linksElife / PubMed:34323216 / PubMed Central
MethodsEM (tomography)
Structure data

EMDB-12958:
Cryo-tomogram showing the modulation of the K5/K14 keratin network in a keratinocyte ghost cell
Method: EM (tomography)

EMDB-12959:
Cryo-tomogram of the K5/K14 keratin network in a keratinocyte ghost cell
Method: EM (tomography)

Source
  • Mus musculus (house mouse)

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