Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Analysis of subunit folding contribution of three yeast large ribosomal subunit proteins required for stabilisation and processing of intermediate nuclear rRNA precursors.
Journal, issue, pages	PLoS One, Vol. 16, Issue 11, Page e0252497, Year 2021
Publish date	Nov 23, 2021
Authors	Gisela Pöll / Michael Pilsl / Joachim Griesenbeck / Herbert Tschochner / Philipp Milkereit /
PubMed Abstract	In yeast and human cells many of the ribosomal proteins (r-proteins) are required for the stabilisation and productive processing of rRNA precursors. Functional coupling of r-protein assembly with ...In yeast and human cells many of the ribosomal proteins (r-proteins) are required for the stabilisation and productive processing of rRNA precursors. Functional coupling of r-protein assembly with the stabilisation and maturation of subunit precursors potentially promotes the production of ribosomes with defined composition. To further decipher mechanisms of such an intrinsic quality control pathway we analysed here the contribution of three yeast large ribosomal subunit r-proteins rpL2 (uL2), rpL25 (uL23) and rpL34 (eL34) for intermediate nuclear subunit folding steps. Structure models obtained from single particle cryo-electron microscopy analyses provided evidence for specific and hierarchic effects on the stable positioning and remodelling of large ribosomal subunit domains. Based on these structural and previous biochemical data we discuss possible mechanisms of r-protein dependent hierarchic domain arrangement and the resulting impact on the stability of misassembled subunits.
External links	PLoS One / PubMed:34813592 / PubMed Central
Methods	EM (single particle)
Resolution	3.1 - 4.72 Å
Structure data	12866 7of1 EMDB-12866, PDB-7of1: Nog1-TAP associated immature ribosomal particle population A from S. cerevisiae Method: EM (single particle) / Resolution: 3.1 Å 12892 7oh3 EMDB-12892, PDB-7oh3: Nog1-TAP associated immature ribosomal particle population B from S. cerevisiae Method: EM (single particle) / Resolution: 3.4 Å 12904 7ohp EMDB-12904, PDB-7ohp: Nog1-TAP associated immature ribosomal particles from S. cerevisiae after rpL25 expression shut down, population A Method: EM (single particle) / Resolution: 3.9 Å 12905 7ohq EMDB-12905, PDB-7ohq: Nog1-TAP associated immature ribosomal particle population C from S. cerevisiae Method: EM (single particle) / Resolution: 3.1 Å 12906 7ohr EMDB-12906, PDB-7ohr: Nog1-TAP associated immature ribosomal particle population E from S. cerevisiae Method: EM (single particle) / Resolution: 4.72 Å 12907 7ohs EMDB-12907, PDB-7ohs: Nog1-TAP associated immature ribosomal particle population F from S. cerevisiae Method: EM (single particle) / Resolution: 4.38 Å 12908 7oht EMDB-12908, PDB-7oht: Nog1-TAP associated immature ribosomal particles from S. cerevisiae after rpL2 expression shut down, population A Method: EM (single particle) / Resolution: 4.7 Å 12909 7ohu EMDB-12909, PDB-7ohu: Nog1-TAP associated immature ribosomal particles from S. cerevisiae after rpL2 expression shut down, population B Method: EM (single particle) / Resolution: 3.7 Å 12910 7ohv EMDB-12910, PDB-7ohv: Nog1-TAP associated immature ribosomal particles from S. cerevisiae after rpL2 expression shut down, population C Method: EM (single particle) / Resolution: 3.9 Å 12911 7ohw EMDB-12911, PDB-7ohw: Nog1-TAP associated immature ribosomal particles from S. cerevisiae after rpL25 expression shut down, population B Method: EM (single particle) / Resolution: 3.5 Å 12912 7ohx EMDB-12912, PDB-7ohx: Nog1-TAP associated immature ribosomal particles from S. cerevisiae after rpL34 expression shut down, population A Method: EM (single particle) / Resolution: 3.3 Å 12913 7ohy EMDB-12913, PDB-7ohy: Nog1-TAP associated immature ribosomal particles from S. cerevisiae after rpL34 expression shut down, population B Method: EM (single particle) / Resolution: 3.9 Å
Chemicals	ChemComp-MG: Unknown entry ChemComp-ZN: Unknown entry
Source	saccharomyces cerevisiae s288c (yeast) saccharomyces cerevisiae (strain atcc 204508 / s288c) (yeast) Baker's yeast (brewer's yeast)
Keywords	RIBOSOME / ribosomal assembly state