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TitleIn-cell structures of conserved supramolecular protein arrays at the mitochondria-cytoskeleton interface in mammalian sperm.
Journal, issue, pagesProc Natl Acad Sci U S A, Vol. 118, Issue 45, Year 2021
Publish dateNov 9, 2021
AuthorsMiguel Ricardo Leung / Riccardo Zenezini Chiozzi / Marc C Roelofs / Johannes F Hevler / Ravi Teja Ravi / Paula Maitan / Min Zhang / Heiko Henning / Elizabeth G Bromfield / Stuart C Howes / Bart M Gadella / Albert J R Heck / Tzviya Zeev-Ben-Mordehai /
PubMed AbstractMitochondria-cytoskeleton interactions modulate cellular physiology by regulating mitochondrial transport, positioning, and immobilization. However, there is very little structural information ...Mitochondria-cytoskeleton interactions modulate cellular physiology by regulating mitochondrial transport, positioning, and immobilization. However, there is very little structural information defining mitochondria-cytoskeleton interfaces in any cell type. Here, we use cryofocused ion beam milling-enabled cryoelectron tomography to image mammalian sperm, where mitochondria wrap around the flagellar cytoskeleton. We find that mitochondria are tethered to their neighbors through intermitochondrial linkers and are anchored to the cytoskeleton through ordered arrays on the outer mitochondrial membrane. We use subtomogram averaging to resolve in-cell structures of these arrays from three mammalian species, revealing they are conserved across species despite variations in mitochondrial dimensions and cristae organization. We find that the arrays consist of boat-shaped particles anchored on a network of membrane pores whose arrangement and dimensions are consistent with voltage-dependent anion channels. Proteomics and in-cell cross-linking mass spectrometry suggest that the conserved arrays are composed of glycerol kinase-like proteins. Ordered supramolecular assemblies may serve to stabilize similar contact sites in other cell types in which mitochondria need to be immobilized in specific subcellular environments, such as in muscles and neurons.
External linksProc Natl Acad Sci U S A / PubMed:34737233 / PubMed Central
MethodsEM (subtomogram averaging)
Resolution24.0 - 35.0 Å
Structure data

EMDB-12354:
ordered protein arrays on the outer mitochondrial membrane of mouse sperm mitochondria
Method: EM (subtomogram averaging) / Resolution: 24.0 Å

EMDB-12355:
ordered protein arrays on the outer mitochondrial membrane of horse sperm mitochondria
Method: EM (subtomogram averaging) / Resolution: 35.0 Å

EMDB-12356:
ordered protein arrays on the outer mitochondrial membrane of pig sperm mitochondria
Method: EM (subtomogram averaging) / Resolution: 35.0 Å

12357

7nie

EMDB-12357: ordered protein arrays on the axoneme-facing surface of the outer mitochondrial membrane in pig sperm mitochondria (alignment focused on one unit)
PDB-7nie: putative glycerol kinase-like proteins anchored on an array of voltage dependent anion channels in the outer mitochondrial membrane of pig sperm mitochondria
Method: EM (subtomogram averaging) / Resolution: 35.0 Å

Source
  • Mus musculus (house mouse)
  • Equus caballus (horse)
  • sus scrofa (pig)
  • Pig (pig)
KeywordsMEMBRANE PROTEIN / mitochondria / sperm

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