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TitleVariety of size and form of GRM2 bacterial microcompartment particles.
Journal, issue, pagesProtein Sci, Vol. 30, Issue 5, Page 1035-1043, Year 2021
Publish dateApr 2, 2021
AuthorsEva Emilija Cesle / Anatolij Filimonenko / Kaspars Tars / Gints Kalnins /
PubMed AbstractBacterial microcompartments (BMCs) are bacterial organelles involved in enzymatic processes, such as carbon fixation, choline, ethanolamine and propanediol degradation, and others. Formed of a semi- ...Bacterial microcompartments (BMCs) are bacterial organelles involved in enzymatic processes, such as carbon fixation, choline, ethanolamine and propanediol degradation, and others. Formed of a semi-permeable protein shell and an enzymatic core, they can enhance enzyme performance and protect the cell from harmful intermediates. With the ability to encapsulate non-native enzymes, BMCs show high potential for applied use. For this goal, a detailed look into shell form variability is significant to predict shell adaptability. Here we present four novel 3D cryo-EM maps of recombinant Klebsiella pneumoniae GRM2 BMC shell particles with the resolution in range of 9 to 22 Å and nine novel 2D classes corresponding to discrete BMC shell forms. These structures reveal icosahedral, elongated, oblate, multi-layered and polyhedral traits of BMCs, indicating considerable variation in size and form as well as adaptability during shell formation processes.
External linksProtein Sci / PubMed:33763934 / PubMed Central
MethodsEM (single particle)
Resolution9.0 - 22.0 Å
Structure data

EMDB-12252:
pT=7 laevo quasi-symmetric bacterial microcompartment particle
Method: EM (single particle) / Resolution: 9.0 Å

EMDB-12253:
D3 fusion of pT=4 quasi-symmetric bacterial microcompartment particles
Method: EM (single particle) / Resolution: 22.0 Å

EMDB-12254:
T=4, Q=8 quasi-symmetric bacterial microcompartment particle
Method: EM (single particle) / Resolution: 17.0 Å

EMDB-12255:
pT=4, Q=10 quasi-symmetric bacterial microcompartment particle
Method: EM (single particle) / Resolution: 17.0 Å

Source
  • Klebsiella pneumoniae (bacteria)

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