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Structure paper

TitleSnapshots of native pre-50S ribosomes reveal a biogenesis factor network and evolutionary specialization.
Journal, issue, pagesMol Cell, Vol. 81, Issue 6, Page 1200-11215.e9, Year 2021
Publish dateMar 18, 2021
AuthorsRainer Nikolay / Tarek Hilal / Sabine Schmidt / Bo Qin / David Schwefel / Carlos H Vieira-Vieira / Thorsten Mielke / Jörg Bürger / Justus Loerke / Kazuaki Amikura / Timo Flügel / Takuya Ueda / Matthias Selbach / Elke Deuerling / Christian M T Spahn /
PubMed AbstractRibosome biogenesis is a fundamental multi-step cellular process that culminates in the formation of ribosomal subunits, whose production and modification are regulated by numerous biogenesis factors. ...Ribosome biogenesis is a fundamental multi-step cellular process that culminates in the formation of ribosomal subunits, whose production and modification are regulated by numerous biogenesis factors. In this study, we analyze physiologic prokaryotic ribosome biogenesis by isolating bona fide pre-50S subunits from an Escherichia coli strain with the biogenesis factor ObgE, affinity tagged at its native gene locus. Our integrative structural approach reveals a network of interacting biogenesis factors consisting of YjgA, RluD, RsfS, and ObgE on the immature pre-50S subunit. In addition, our study provides mechanistic insight into how the GTPase ObgE, in concert with other biogenesis factors, facilitates the maturation of the 50S functional core and reveals both conserved and divergent evolutionary features of ribosome biogenesis between prokaryotes and eukaryotes.
External linksMol Cell / PubMed:33639093
MethodsEM (single particle)
Resolution2.4 - 4.0 Å
Structure data

12215

7bl2

EMDB-12215, PDB-7bl2:
pre-50S-ObgE particle state 1
Method: EM (single particle) / Resolution: 3.7 Å

12216

7bl3

EMDB-12216, PDB-7bl3:
pre-50S-ObgE particle state 2
Method: EM (single particle) / Resolution: 3.5 Å

12217

7bl4

EMDB-12217, PDB-7bl4:
in vitro reconstituted 50S-ObgE-GMPPNP-RsfS particle
Method: EM (single particle) / Resolution: 2.4 Å

12218

7bl5

EMDB-12218, PDB-7bl5:
pre-50S-ObgE particle
Method: EM (single particle) / Resolution: 3.3 Å

12219

7bl6

EMDB-12219, PDB-7bl6:
50S-ObgE-GMPPNP particle
Method: EM (single particle) / Resolution: 4.0 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-ZN:
Unknown entry

ChemComp-HOH:
WATER / Water

ChemComp-GNP:
PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER / GppNHp, GMPPNP, energy-carrying molecule analogue*YM / 5'-Guanylyl imidodiphosphate

ChemComp-GDP:
GUANOSINE-5'-DIPHOSPHATE / GDP, energy-carrying molecule*YM / Guanosine diphosphate

Source
  • escherichia coli (strain k12) (bacteria)
  • escherichia coli str. k-12 substr. mg1655 (bacteria)
  • escherichia coli (E. coli)
KeywordsRIBOSOME / pre-50S / ribosome biogenesis / ribosome assembly

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