Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Cryo-EM photosystem I structure reveals adaptation mechanisms to extreme high light in Chlorella ohadii.
Journal, issue, pages	Nat Plants, Vol. 7, Issue 9, Page 1314-1322, Year 2021
Publish date	Aug 30, 2021
Authors	Ido Caspy / Ehud Neumann / Maria Fadeeva / Varda Liveanu / Anton Savitsky / Anna Frank / Yael Levi Kalisman / Yoel Shkolnisky / Omer Murik / Haim Treves / Volker Hartmann / Marc M Nowaczyk / Wolfgang Schuhmann / Matthias Rögner / Itamar Willner / Aaron Kaplan / Gadi Schuster / Nathan Nelson / Wolfgang Lubitz / Rachel Nechushtai /
PubMed Abstract	Photosynthesis in deserts is challenging since it requires fast adaptation to rapid night-to-day changes, that is, from dawn's low light (LL) to extreme high light (HL) intensities during the daytime. ...Photosynthesis in deserts is challenging since it requires fast adaptation to rapid night-to-day changes, that is, from dawn's low light (LL) to extreme high light (HL) intensities during the daytime. To understand these adaptation mechanisms, we purified photosystem I (PSI) from Chlorella ohadii, a green alga that was isolated from a desert soil crust, and identified the essential functional and structural changes that enable the photosystem to perform photosynthesis under extreme high light conditions. The cryo-electron microscopy structures of PSI from cells grown under low light (PSI) and high light (PSI), obtained at 2.70 and 2.71 Å, respectively, show that part of light-harvesting antenna complex I (LHCI) and the core complex subunit (PsaO) are eliminated from PSI to minimize the photodamage. An additional change is in the pigment composition and their number in LHCI; about 50% of chlorophyll b is replaced by chlorophyll a. This leads to higher electron transfer rates in PSI and might enable C. ohadii PSI to act as a natural photosynthesiser in photobiocatalytic systems. PSI or PSI were attached to an electrode and their induced photocurrent was determined. To obtain photocurrents comparable with PSI, 25 times the amount of PSI was required, demonstrating the high efficiency of PSI. Hence, we suggest that C. ohadii PSI is an ideal candidate for the design of desert artificial photobiocatalytic systems.
External links	Nat Plants / PubMed:34462576
Methods	EM (single particle)
Resolution	2.7 - 4.2 Å
Structure data	11588 6zzx EMDB-11588, PDB-6zzx: Structure of low-light grown Chlorella ohadii Photosystem I Method: EM (single particle) / Resolution: 2.7 Å 11589 6zzy EMDB-11589, PDB-6zzy: Structure of high-light grown Chlorella ohadii photosystem I Method: EM (single particle) / Resolution: 3.16 Å 11640 7a4p EMDB-11640, PDB-7a4p: Structure of small high-light grown Chlorella ohadii photosystem I Method: EM (single particle) / Resolution: 4.2 Å
Chemicals	ChemComp-CL0: CHLOROPHYLL A ISOMER / Chlorophyll a ChemComp-CLA: CHLOROPHYLL A / Chlorophyll a ChemComp-CHL: CHLOROPHYLL B / Chlorophyll b ChemComp-PQN: PHYLLOQUINONE / Phytomenadione ChemComp-SF4: IRON/SULFUR CLUSTER / Iron–sulfur cluster ChemComp-BCR: BETA-CAROTENE / Β-Carotene ChemComp-LHG: 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE / phospholipidYM / Phosphatidylglycerol ChemComp-LMG: 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE ChemComp-SQD: 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL ChemComp-PTY: PHOSPHATIDYLETHANOLAMINE / phospholipidYM / Phosphatidylethanolamine ChemComp-3PH: 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE / Phosphatidic acid ChemComp-LMT: DODECYL-BETA-D-MALTOSIDE / detergentYM ChemComp-DGD: DIGALACTOSYL DIACYL GLYCEROL (DGDG) ChemComp-PCW: 1,2-DIOLEOYL-SN-GLYCERO-3-PHOSPHOCHOLINE / DOPC, phospholipidYM ChemComp-LPX: (2S)-3-{[(R)-(2-aminoethoxy)(hydroxy)phosphoryl]oxy}-2-hydroxypropyl hexadecanoate ChemComp-NEX: (1R,3R)-6-{(3E,5E,7E,9E,11E,13E,15E,17E)-18-[(1S,4R,6R)-4-HYDROXY-2,2,6-TRIMETHYL-7-OXABICYCLO[4.1.0]HEPT-1-YL]-3,7,12,16-TETRAMETHYLOCTADECA-1,3,5,7,9,11,13,15,17-NONAENYLIDENE}-1,5,5-TRIMETHYLCYCLOHEXANE-1,3-DIOL ChemComp-RRX: (3R)-beta,beta-caroten-3-ol / Β-Cryptoxanthin ChemComp-SPH: SPHINGOSINE / Sphingosine ChemComp-ECH: beta,beta-caroten-4-one / Echinenone ChemComp-LUT: (3R,3'R,6S)-4,5-DIDEHYDRO-5,6-DIHYDRO-BETA,BETA-CAROTENE-3,3'-DIOL / Lutein ChemComp-AXT: ASTAXANTHIN / Astaxanthin ChemComp-OLA: OLEIC ACID / Oleic acid ChemComp-QTB: (3~{E},5~{E},7~{E})-6-methyl-8-[(6~{R})-2,2,6-trimethylcyclohexyl]octa-3,5,7-trien-2-one ChemComp-PLM: PALMITIC ACID / Palmitic acid ChemComp-DGA: DIACYL GLYCEROL / Diglyceride ChemComp-13X: benzene-1,3,5-triol / Phloroglucinol ChemComp-XAT: (3S,5R,6S,3'S,5'R,6'S)-5,6,5',6'-DIEPOXY-5,6,5',6'- TETRAHYDRO-BETA,BETA-CAROTENE-3,3'-DIOL / Violaxanthin ChemComp-4RF: Tripalmitoylglycerol / Tripalmitin ChemComp-P5S: O-[(R)-{[(2R)-2,3-bis(octadecanoyloxy)propyl]oxy}(hydroxy)phosphoryl]-L-serine / Phosphatidylserine ChemComp-LAP: [2-((1-OXODODECANOXY-(2-HYDROXY-3-PROPANYL))-PHOSPHONATE-OXY)-ETHYL]-TRIMETHYLAMMONIUM ChemComp-HOH: WATER / Water ChemComp-ERG: ERGOSTEROL / Ergosterol ChemComp-GG0: 2-(2-azanylethanoylamino)ethanoic acid / pH bufferYM / Glycylglycine ChemComp-C7Z: (1~{S})-3,5,5-trimethyl-4-[(1~{E},3~{E},5~{E},7~{E},9~{E},11~{E},13~{E},15~{E},17~{E})-3,7,12,16-tetramethyl-18-[(4~{S})-2,6,6-trimethyl-4-oxidanyl-cyclohexen-1-yl]octadeca-1,3,5,7,9,11,13,15,17-nonaenyl]cyclohex-3-en-1-ol ChemComp-A8S: (2Z,4E)-5-[(1S)-1-hydroxy-2,6,6-trimethyl-4-oxocyclohex-2-en-1-yl]-3-methylpenta-2,4-dienoic acid / hormoneYM / Abscisic acid
Source	chlorella ohadii (plant) Freshwater green alga (plant)
Keywords	PHOTOSYNTHESIS / photosystem I / light stress