Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Novel features of centriole polarity and cartwheel stacking revealed by cryo-tomography.
Journal, issue, pages	EMBO J, Vol. 39, Issue 22, Page e106249, Year 2020
Publish date	Nov 16, 2020
Authors	Sergey Nazarov / Alexandra Bezler / Georgios N Hatzopoulos / Veronika Nemčíková Villímová / Davide Demurtas / Maeva Le Guennec / Paul Guichard / Pierre Gönczy /
PubMed Abstract	Centrioles are polarized microtubule-based organelles that seed the formation of cilia, and which assemble from a cartwheel containing stacked ring oligomers of SAS-6 proteins. A cryo-tomography map ...Centrioles are polarized microtubule-based organelles that seed the formation of cilia, and which assemble from a cartwheel containing stacked ring oligomers of SAS-6 proteins. A cryo-tomography map of centrioles from the termite flagellate Trichonympha spp. was obtained previously, but higher resolution analysis is likely to reveal novel features. Using sub-tomogram averaging (STA) in T. spp. and Trichonympha agilis, we delineate the architecture of centriolar microtubules, pinhead, and A-C linker. Moreover, we report ~25 Å resolution maps of the central cartwheel, revealing notably polarized cartwheel inner densities (CID). Furthermore, STA of centrioles from the distant flagellate Teranympha mirabilis uncovers similar cartwheel architecture and a distinct filamentous CID. Fitting the CrSAS-6 crystal structure into the flagellate maps and analyzing cartwheels generated in vitro indicate that SAS-6 rings can directly stack onto one another in two alternating configurations: with a slight rotational offset and in register. Overall, improved STA maps in three flagellates enabled us to unravel novel architectural features, including of centriole polarity and cartwheel stacking, thus setting the stage for an accelerated elucidation of underlying assembly mechanisms.
External links	EMBO J / PubMed:32954505 / PubMed Central
Methods	EM (subtomogram averaging)
Resolution	23.0 - 40.0 Å
Structure data	EMDB-10916: Sub-tomograms average of the Trichonympha agilis proximal basal body, central part of the cartwheel without variations, 55%. Method: EM (subtomogram averaging) / Resolution: 25.0 Å EMDB-10918: Sub-tomograms average of the Trichonympha agilis proximal basal body, central part of the cartwheel with variations, 45% Method: EM (subtomogram averaging) / Resolution: 23.0 Å EMDB-10922: Sub-tomograms average of the Teranympha mirabilis proximal basal body, central part of the cartwheel without variations, 64% Method: EM (subtomogram averaging) / Resolution: 23.0 Å EMDB-10923: Sub-tomograms average of the Teranympha mirabilis proximal basal body, central part of the cartwheel with variations, 36% Method: EM (subtomogram averaging) / Resolution: 33.0 Å EMDB-10927: Sub-tomograms average of the Trichonympha proximal basal body, central part of the cartwheel Method: EM (subtomogram averaging) / Resolution: 24.0 Å EMDB-10928: Sub-tomograms average of the Chlamydomonas reinhardtii CrSAS-6[NL] in vitro stacked cartwheels Method: EM (subtomogram averaging) / Resolution: 25.0 Å EMDB-10931: Microtubule triplet from Trichonympha agilis Method: EM (subtomogram averaging) / Resolution: 38.0 Å EMDB-10932: Microtubule triplet from Trichonympha spp. Method: EM (subtomogram averaging) / Resolution: 38.0 Å EMDB-10934: Microtubule triplet from Teranympha mirabilis Method: EM (subtomogram averaging) / Resolution: 39.0 Å EMDB-10935: Pinhead of the microtubule triplet of isolated Trichonympha agilis centriole Method: EM (subtomogram averaging) / Resolution: 39.0 Å EMDB-10937: A-C linker connecting microtubule triplets of the Trichonympha agilis centriole Method: EM (subtomogram averaging) / Resolution: 37.0 Å EMDB-10938: Pinhead of the microtubule triplet of the Trichonympha spp. centriole Method: EM (subtomogram averaging) / Resolution: 40.0 Å EMDB-10939: A-C linker connecting microtubule triplets of the Trichonympha spp. centriole Method: EM (subtomogram averaging) / Resolution: 35.0 Å EMDB-10941: Pinhead of the microtubule triplet of the Teranympha mirabilis centriole Method: EM (subtomogram averaging) / Resolution: 37.0 Å EMDB-10942: A-C linker connecting microtubule triplets of the Teranympha mirabilis centriole Method: EM (subtomogram averaging) / Resolution: 36.0 Å
Source	Trichonympha agilis (eukaryote) Teranympha mirabilis (eukaryote) Trichonympha campanula (eukaryote) Chlamydomonas reinhardtii (plant) Trichonympha sp. (eukaryote)