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TitleArchitecture of the centriole cartwheel-containing region revealed by cryo-electron tomography.
Journal, issue, pagesEMBO J, Vol. 39, Issue 22, Page e106246, Year 2020
Publish dateNov 16, 2020
AuthorsNikolai Klena / Maeva Le Guennec / Anne-Marie Tassin / Hugo van den Hoek / Philipp S Erdmann / Miroslava Schaffer / Stefan Geimer / Gabriel Aeschlimann / Lubomir Kovacik / Yashar Sadian / Kenneth N Goldie / Henning Stahlberg / Benjamin D Engel / Virginie Hamel / Paul Guichard /
PubMed AbstractCentrioles are evolutionarily conserved barrels of microtubule triplets that form the core of the centrosome and the base of the cilium. While the crucial role of the proximal region in centriole ...Centrioles are evolutionarily conserved barrels of microtubule triplets that form the core of the centrosome and the base of the cilium. While the crucial role of the proximal region in centriole biogenesis has been well documented, its native architecture and evolutionary conservation remain relatively unexplored. Here, using cryo-electron tomography of centrioles from four evolutionarily distant species, we report on the architectural diversity of the centriole's proximal cartwheel-bearing region. Our work reveals that the cartwheel central hub is constructed from a stack of paired rings with cartwheel inner densities inside. In both Paramecium and Chlamydomonas, the repeating structural unit of the cartwheel has a periodicity of 25 nm and consists of three ring pairs, with 6 radial spokes emanating and merging into a single bundle that connects to the microtubule triplet via the D2-rod and the pinhead. Finally, we identified that the cartwheel is indirectly connected to the A-C linker through the triplet base structure extending from the pinhead. Together, our work provides unprecedented evolutionary insights into the architecture of the centriole proximal region, which underlies centriole biogenesis.
External linksEMBO J / PubMed:32954513 / PubMed Central
MethodsEM (subtomogram averaging)
Resolution31.2 - 37.4 Å
Structure data

EMDB-10726:
Electron cryo-tomography reconstruction and subvolume averaging of the A-C linker from the end of the proximal region of Paramecium tetraurelia basal body
Method: EM (subtomogram averaging) / Resolution: 31.2 Å

EMDB-10727:
Electron cryo-tomography reconstruction and subvolume averaging of the A-C linker from the start of the proximal region of Paramecium tetraurelia basal body
Method: EM (subtomogram averaging) / Resolution: 31.2 Å

EMDB-10728:
Electron cryo-tomography reconstruction and subvolume averaging of the microtubule triplet from the end of the proximal region of Paramecium tetraurelia basal body
Method: EM (subtomogram averaging) / Resolution: 37.4 Å

EMDB-10729:
Electron cryo-tomography reconstruction and subvolume averaging of the microtubule triplet from the start of the proximal region of Paramecium tetraurelia basal body
Method: EM (subtomogram averaging) / Resolution: 31.5 Å

Source
  • Paramecium tetraurelia (eukaryote)

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