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TitleDynamics of uS19 C-Terminal Tail during the Translation Elongation Cycle in Human Ribosomes.
Journal, issue, pagesCell Rep, Vol. 31, Issue 1, Page 107473, Year 2020
Publish dateApr 7, 2020
AuthorsVarun Bhaskar / Alexandra Graff-Meyer / Andreas D Schenk / Simone Cavadini / Ottilie von Loeffelholz / S Kundhavai Natchiar / Caroline G Artus-Revel / Hans-Rudolf Hotz / Gabriel Bretones / Bruno P Klaholz / Jeffrey A Chao /
PubMed AbstractRibosomes undergo multiple conformational transitions during translation elongation. Here, we report the high-resolution cryoelectron microscopy (cryo-EM) structure of the human 80S ribosome in the ...Ribosomes undergo multiple conformational transitions during translation elongation. Here, we report the high-resolution cryoelectron microscopy (cryo-EM) structure of the human 80S ribosome in the post-decoding pre-translocation state (classical-PRE) at 3.3-Å resolution along with the rotated (hybrid-PRE) and the post-translocation states (POST). The classical-PRE state ribosome structure reveals a previously unobserved interaction between the C-terminal region of the conserved ribosomal protein uS19 and the A- and P-site tRNAs and the mRNA in the decoding site. In addition to changes in the inter-subunit bridges, analysis of different ribosomal conformations reveals the dynamic nature of this domain and suggests a role in tRNA accommodation and translocation during elongation. Furthermore, we show that disease-associated mutations in uS19 result in increased frameshifting. Together, this structure-function analysis provides mechanistic insights into the role of the uS19 C-terminal tail in the context of mammalian ribosomes.
External linksCell Rep / PubMed:32268098
MethodsEM (single particle)
Resolution3.0 - 3.5 Å
Structure data

10668

6y0g

EMDB-10668, PDB-6y0g:
Structure of human ribosome in classical-PRE state
Method: EM (single particle) / Resolution: 3.2 Å

10674

6y2l

EMDB-10674: Cryo-EM map of human ribosome in POST state
PDB-6y2l: Structure of human ribosome in POST state
Method: EM (single particle) / Resolution: 3.0 Å

10690

6y57

EMDB-10690: Cryo-EM map of human ribosome in hybrid-PRE state
PDB-6y57: Structure of human ribosome in hybrid-PRE state
Method: EM (single particle) / Resolution: 3.5 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-3HE:
4-{(2R)-2-[(1S,3S,5S)-3,5-dimethyl-2-oxocyclohexyl]-2-hydroxyethyl}piperidine-2,6-dione / Cycloheximide

ChemComp-ZN:
Unknown entry

ChemComp-HOH:
WATER / Water

Source
  • homo sapiens (human)
  • Human (human)
KeywordsRIBOSOME / Classical-PRE state / Cycloheximide / POST state / hybrid-PRE state

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