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TitleVisualization of release factor 3 on the ribosome during termination of protein synthesis.
Journal, issue, pagesNature, Vol. 427, Issue 6977, Page 862-865, Year 2004
Publish dateFeb 26, 2004
AuthorsBruno P Klaholz / Alexander G Myasnikov / Marin Van Heel /
PubMed AbstractTermination of protein synthesis by the ribosome requires two release factor (RF) classes. The class II RF3 is a GTPase that removes class I RFs (RF1 or RF2) from the ribosome after release of the ...Termination of protein synthesis by the ribosome requires two release factor (RF) classes. The class II RF3 is a GTPase that removes class I RFs (RF1 or RF2) from the ribosome after release of the nascent polypeptide. RF3 in the GDP state binds to the ribosomal class I RF complex, followed by an exchange of GDP for GTP and release of the class I RF. As GTP hydrolysis triggers release of RF3 (ref. 4), we trapped RF3 on Escherichia coli ribosomes using a nonhydrolysable GTP analogue. Here we show by cryo-electron microscopy that the complex can adopt two different conformational states. In 'state 1', RF3 is pre-bound to the ribosome, whereas in 'state 2' RF3 contacts the ribosome GTPase centre. The transfer RNA molecule translocates from the peptidyl site in state 1 to the exit site in state 2. This translocation is associated with a large conformational rearrangement of the ribosome. Because state 1 seems able to accommodate simultaneously both RF3 and RF2, whose position is known from previous studies, we can infer the release mechanism of class I RFs.
External linksNature / PubMed:14985767
MethodsEM (single particle)
Resolution17.5 - 17.9 Å
Structure data

EMDB-1064:
Visualization of release factor 3 on the ribosome during termination of protein synthesis.
Method: EM (single particle) / Resolution: 17.9 Å

EMDB-1065:
Visualization of release factor 3 on the ribosome during termination of protein synthesis.
Method: EM (single particle) / Resolution: 17.5 Å

Source
  • Escherichia coli (E. coli)

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