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TitleCryo-electron microscopy reveals two distinct type IV pili assembled by the same bacterium.
Journal, issue, pagesNat Commun, Vol. 11, Issue 1, Page 2231, Year 2020
Publish dateMay 6, 2020
AuthorsAlexander Neuhaus / Muniyandi Selvaraj / Ralf Salzer / Julian D Langer / Kerstin Kruse / Lennart Kirchner / Kelly Sanders / Bertram Daum / Beate Averhoff / Vicki A M Gold /
PubMed AbstractType IV pili are flexible filaments on the surface of bacteria, consisting of a helical assembly of pilin proteins. They are involved in bacterial motility (twitching), surface adhesion, biofilm ...Type IV pili are flexible filaments on the surface of bacteria, consisting of a helical assembly of pilin proteins. They are involved in bacterial motility (twitching), surface adhesion, biofilm formation and DNA uptake (natural transformation). Here, we use cryo-electron microscopy and mass spectrometry to show that the bacterium Thermus thermophilus produces two forms of type IV pilus ('wide' and 'narrow'), differing in structure and protein composition. Wide pili are composed of the major pilin PilA4, while narrow pili are composed of a so-far uncharacterized pilin which we name PilA5. Functional experiments indicate that PilA4 is required for natural transformation, while PilA5 is important for twitching motility.
External linksNat Commun / PubMed:32376942 / PubMed Central
MethodsEM (helical sym.)
Resolution3.22 - 3.49 Å
Structure data

10647

6xxd

EMDB-10647: CryoEM structure of the wide type IV pilus (PilA4) from Thermus thermophilus
PDB-6xxd: CryoEM structure of the type IV pilin PilA4 from Thermus thermophilus
Method: EM (helical sym.) / Resolution: 3.22 Å

10648

6xxe

EMDB-10648: CryoEM structure of the narrow type IV pilus (PilA5) from Thermus thermophilus
PDB-6xxe: CryoEM structure of the type IV pilin PilA5 from Thermus thermophilus
Method: EM (helical sym.) / Resolution: 3.49 Å

Source
  • Thermus thermophilus HB27 (bacteria)
  • thermus thermophilus (strain hb27 / atcc baa-163 / dsm 7039) (bacteria)
KeywordsCELL ADHESION / Type IV pilin glycosylation twitching motility

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