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TitleAmbidextrous helical nanotubes from self-assembly of designed helical hairpin motifs.
Journal, issue, pagesProc Natl Acad Sci U S A, Vol. 116, Issue 29, Page 14456-14464, Year 2019
Publish dateJul 16, 2019
AuthorsSpencer A Hughes / Fengbin Wang / Shengyuan Wang / Mark A B Kreutzberger / Tomasz Osinski / Albina Orlova / Joseph S Wall / Xiaobing Zuo / Edward H Egelman / Vincent P Conticello /
PubMed AbstractTandem repeat proteins exhibit native designability and represent potentially useful scaffolds for the construction of synthetic biomimetic assemblies. We have designed 2 synthetic peptides, HEAT_R1 ...Tandem repeat proteins exhibit native designability and represent potentially useful scaffolds for the construction of synthetic biomimetic assemblies. We have designed 2 synthetic peptides, HEAT_R1 and LRV_M3Δ1, based on the consensus sequences of single repeats of thermophilic HEAT (PBS_HEAT) and Leucine-Rich Variant (LRV) structural motifs, respectively. Self-assembly of the peptides afforded high-aspect ratio helical nanotubes. Cryo-electron microscopy with direct electron detection was employed to analyze the structures of the solvated filaments. The 3D reconstructions from the cryo-EM maps led to atomic models for the HEAT_R1 and LRV_M3Δ1 filaments at resolutions of 6.0 and 4.4 Å, respectively. Surprisingly, despite sequence similarity at the lateral packing interface, HEAT_R1 and LRV_M3Δ1 filaments adopt the opposite helical hand and differ significantly in helical geometry, while retaining a local conformation similar to previously characterized repeat proteins of the same class. The differences in the 2 filaments could be rationalized on the basis of differences in cohesive interactions at the lateral and axial interfaces. These structural data reinforce previous observations regarding the structural plasticity of helical protein assemblies and the need for high-resolution structural analysis. Despite these observations, the native designability of tandem repeat proteins offers the opportunity to engineer novel helical nanotubes. Moreover, the resultant nanotubes have independently addressable and chemically distinguishable interior and exterior surfaces that would facilitate applications in selective recognition, transport, and release.
External linksProc Natl Acad Sci U S A / PubMed:31262809 / PubMed Central
MethodsEM (helical sym.)
Resolution4.4 - 6.0 Å
Structure data

0252

6hqe

EMDB-0252, PDB-6hqe:
Cryo-EM of self-assembly peptide filament LRV_M3delta1
Method: EM (helical sym.) / Resolution: 4.4 Å

9136

6mk1

EMDB-9136, PDB-6mk1:
Cryo-EM of self-assembly peptide filament HEAT_R1
Method: EM (helical sym.) / Resolution: 6.0 Å

Source
  • azotobacter vinelandii (bacteria)
  • methanothermobacter thermautotrophicus (archaea)
KeywordsPROTEIN FIBRIL / filament / self-assembly peptide filament / Cryo-EM

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