EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural basis for phage-mediated activation and repression of bacterial DSR2 anti-phage defense system.
ジャーナル・号・ページ	Nat Commun, Vol. 15, Issue 1, Page 2797, Year 2024
掲載日	2024年3月30日
著者	Jun-Tao Zhang / Xiao-Yu Liu / Zhuolin Li / Xin-Yang Wei / Xin-Yi Song / Ning Cui / Jirui Zhong / Hongchun Li / Ning Jia /
PubMed 要旨	Silent information regulator 2 (Sir2) proteins typically catalyze NAD-dependent protein deacetylation. The recently identified bacterial Sir2 domain-containing protein, defense-associated sirtuin 2 ...Silent information regulator 2 (Sir2) proteins typically catalyze NAD-dependent protein deacetylation. The recently identified bacterial Sir2 domain-containing protein, defense-associated sirtuin 2 (DSR2), recognizes the phage tail tube and depletes NAD to abort phage propagation, which is counteracted by the phage-encoded DSR anti-defense 1 (DSAD1), but their molecular mechanisms remain unclear. Here, we determine cryo-EM structures of inactive DSR2 in its apo form, DSR2-DSAD1 and DSR2-DSAD1-NAD, as well as active DSR2-tube and DSR2-tube-NAD complexes. DSR2 forms a tetramer with its C-terminal sensor domains (CTDs) in two distinct conformations: CTD or CTD. Monomeric, rather than oligomeric, tail tube proteins preferentially bind to CTD and activate Sir2 for NAD hydrolysis. DSAD1 binding to CTD allosterically inhibits tube binding and tube-mediated DSR2 activation. Our findings provide mechanistic insight into DSR2 assembly, tube-mediated DSR2 activation, and DSAD1-mediated inhibition and NAD substrate catalysis in bacterial DSR2 anti-phage defense systems.
リンク	Nat Commun / PubMed:38555355 / PubMed Central
手法	EM (単粒子)
解像度	2.49 - 3.62 Å
構造データ	37919 8wy8 EMDB-37919, PDB-8wy8: Cryo-EM structure of DSR2 apo complex 手法: EM (単粒子) / 解像度: 3.1 Å 37920 8wy9 EMDB-37920, PDB-8wy9: Cryo-EM structure of DSR2 apo (partial) complex 手法: EM (単粒子) / 解像度: 2.57 Å 37921 8wya EMDB-37921, PDB-8wya: Cryo-EM structure of DSR2-tube complex 手法: EM (単粒子) / 解像度: 3.62 Å 37922 8wyb EMDB-37922, PDB-8wyb: Cryo-EM structure of DSR2 (H171A)-tube-NAD+ complex 手法: EM (単粒子) / 解像度: 3.37 Å 37923 8wyc EMDB-37923, PDB-8wyc: Cryo-EM structure of DSR2 (H171A)-tube-NAD+ (partial) complex 手法: EM (単粒子) / 解像度: 3.0 Å 37924 8wyd EMDB-37924, PDB-8wyd: Cryo-EM structure of DSR2-DSAD1 complex 手法: EM (単粒子) / 解像度: 2.56 Å 37925 8wye EMDB-37925, PDB-8wye: Cryo-EM structure of DSR2-DSAD1 (partial) complex 手法: EM (単粒子) / 解像度: 2.49 Å 37926 8wyf EMDB-37926, PDB-8wyf: Cryo-EM structure of DSR2-DSAD1-NAD+ (partial) complex 手法: EM (単粒子) / 解像度: 2.85 Å
化合物	ChemComp-NAD: NICOTINAMIDE-ADENINE-DINUCLEOTIDE / NAD*YM / ニコチンアミドアデニンジヌクレオチド
由来	bacillus subtilis (枯草菌) bacillus phage spbc2 (ファージ) bacillus phage spr (ファージ)
キーワード	ANTIVIRAL PROTEIN / Phage defense proteins