EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structure of full-length ERGIC-53 in complex with MCFD2 for cargo transport.
ジャーナル・号・ページ	Nat Commun, Vol. 15, Issue 1, Page 2404, Year 2024
掲載日	2024年3月16日
著者	Satoshi Watanabe / Yoshiaki Kise / Kento Yonezawa / Mariko Inoue / Nobutaka Shimizu / Osamu Nureki / Kenji Inaba /
PubMed 要旨	ERGIC-53 transports certain subsets of newly synthesized secretory proteins and membrane proteins from the endoplasmic reticulum to the Golgi apparatus. Despite numerous structural and functional ...ERGIC-53 transports certain subsets of newly synthesized secretory proteins and membrane proteins from the endoplasmic reticulum to the Golgi apparatus. Despite numerous structural and functional studies since its identification, the overall architecture and mechanism of action of ERGIC-53 remain unclear. Here we present cryo-EM structures of full-length ERGIC-53 in complex with its functional partner MCFD2. These structures reveal that ERGIC-53 exists as a homotetramer, not a homohexamer as previously suggested, and comprises a four-leaf clover-like head and a long stalk composed of three sets of four-helix coiled-coil followed by a transmembrane domain. 3D variability analysis visualizes the flexible motion of the long stalk and local plasticity of the head region. Notably, MCFD2 is shown to possess a Zn-binding site in its N-terminal lid, which appears to modulate cargo binding. Altogether, distinct mechanisms of cargo capture and release by ERGIC- 53 via the stalk bending and metal binding are proposed.
リンク	Nat Commun / PubMed:38493152 / PubMed Central
手法	EM (単粒子)
解像度	2.53 - 6.76 Å
構造データ	36467 8jp4 EMDB-36467, PDB-8jp4: Cryo-EM structure of the head region of full-length ERGIC-53 with MCFD2 (form A) 手法: EM (単粒子) / 解像度: 2.53 Å 36468 8jp5 EMDB-36468, PDB-8jp5: Cryo-EM structures of the head region of full-length ERGIC-53 with MCFD2 (form B) 手法: EM (単粒子) / 解像度: 2.59 Å 36469 8jp6 EMDB-36469, PDB-8jp6: Cryo-EM structures of the head region of full-length ERGIC-53 with MCFD2 (Substate A) 手法: EM (単粒子) / 解像度: 3.29 Å 36470 8jp7 EMDB-36470, PDB-8jp7: Cryo-EM structure of the head region of full-length ERGIC-53 with MCFD2 (Substate B) 手法: EM (単粒子) / 解像度: 3.51 Å 36471 8jp8 EMDB-36471, PDB-8jp8: Cryo-EM structure of the head region of full-length ERGIC-53 with MCFD2 (Substate C) 手法: EM (単粒子) / 解像度: 3.39 Å 36472 8jp9 EMDB-36472, PDB-8jp9: Cryo-EM structure of the head region of full-length ERGIC-53 with MCFD2 (Substate D) 手法: EM (単粒子) / 解像度: 3.37 Å 36479 8jpg EMDB-36479, PDB-8jpg: Cryo-EM structure of full-length ERGIC-53 with MCFD2 手法: EM (単粒子) / 解像度: 6.76 Å EMDB-36482: cryoEM structure of ERGIC-53 deltaH34 mutant with MCFD2 手法: EM (単粒子) / 解像度: 3.78 Å
化合物	ChemComp-CA: Unknown entry / カルシウムジカチオン ChemComp-ZN: Unknown entry
由来	homo sapiens (ヒト)
キーワード	PROTEIN TRANSPORT / cargo receptor / calcium (カルシウム) / zinc (亜鉛)