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-Structure paper
タイトル | Cryo-EM structures of RNA polymerase II-nucleosome complexes rewrapping transcribed DNA. |
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ジャーナル・号・ページ | J Biol Chem, Vol. 299, Issue 12, Page 105477, Year 2023 |
掲載日 | 2023年11月17日 |
著者 | Munetaka Akatsu / Haruhiko Ehara / Tomoya Kujirai / Risa Fujita / Tomoko Ito / Ken Osumi / Mitsuo Ogasawara / Yoshimasa Takizawa / Shun-Ichi Sekine / Hitoshi Kurumizaka / |
PubMed 要旨 | RNA polymerase II (RNAPII) transcribes DNA wrapped in the nucleosome by stepwise pausing, especially at nucleosomal superhelical locations -5 and -1 [SHL(-5) and SHL(-1), respectively]. In the ...RNA polymerase II (RNAPII) transcribes DNA wrapped in the nucleosome by stepwise pausing, especially at nucleosomal superhelical locations -5 and -1 [SHL(-5) and SHL(-1), respectively]. In the present study, we performed cryo-electron microscopy analyses of RNAPII-nucleosome complexes paused at a major nucleosomal pausing site, SHL(-1). We determined two previously undetected structures, in which the transcribed DNA behind RNAPII is sharply kinked at the RNAPII exit tunnel and rewrapped around the nucleosomal histones in front of RNAPII by DNA looping. This DNA kink shifts the DNA orientation toward the nucleosome, and the transcribed DNA region interacts with basic amino acid residues of histones H2A, H2B, and H3 exposed by the RNAPII-mediated nucleosomal DNA peeling. The DNA loop structure was not observed in the presence of the transcription elongation factors Spt4/5 and Elf1. These RNAPII-nucleosome structures provide important information for understanding the functional relevance of DNA looping during transcription elongation in the nucleosome. |
リンク | J Biol Chem / PubMed:37981206 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 3.2 - 6.7 Å |
構造データ | EMDB-36251, PDB-8jh2: EMDB-36252, PDB-8jh3: EMDB-36253, PDB-8jh4: EMDB-37848: RNA polymerase II elongation complex bound with Elf1, Spt4/5 and foreign DNA, stalled at SHL(0) of the nucleosome |
化合物 | ChemComp-ZN: ChemComp-MG: |
由来 |
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キーワード | Transcription/DNA/RNA / Transcription-DNA-RNA complex / RNAPII / Cryo-EM (低温電子顕微鏡法) |