EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Cryo-EM structures of human zinc transporter ZnT7 reveal the mechanism of Zn uptake into the Golgi apparatus.
ジャーナル・号・ページ	Nat Commun, Vol. 14, Issue 1, Page 4770, Year 2023
掲載日	2023年8月8日
著者	Han Ba Bui / Satoshi Watanabe / Norimichi Nomura / Kehong Liu / Tomoko Uemura / Michio Inoue / Akihisa Tsutsumi / Hiroyuki Fujita / Kengo Kinoshita / Yukinari Kato / So Iwata / Masahide Kikkawa / Kenji Inaba /
PubMed 要旨	Zinc ions (Zn) are vital to most cells, with the intracellular concentrations of Zn being tightly regulated by multiple zinc transporters located at the plasma and organelle membranes. We herein ...Zinc ions (Zn) are vital to most cells, with the intracellular concentrations of Zn being tightly regulated by multiple zinc transporters located at the plasma and organelle membranes. We herein present the 2.2-3.1 Å-resolution cryo-EM structures of a Golgi-localized human Zn/H antiporter ZnT7 (hZnT7) in Zn-bound and unbound forms. Cryo-EM analyses show that hZnT7 exists as a dimer via tight interactions in both the cytosolic and transmembrane (TM) domains of two protomers, each of which contains a single Zn-binding site in its TM domain. hZnT7 undergoes a TM-helix rearrangement to create a negatively charged cytosolic cavity for Zn entry in the inward-facing conformation and widens the luminal cavity for Zn release in the outward-facing conformation. An exceptionally long cytosolic histidine-rich loop characteristic of hZnT7 binds two Zn ions, seemingly facilitating Zn recruitment to the TM metal transport pathway. These structures permit mechanisms of hZnT7-mediated Zn uptake into the Golgi to be proposed.
リンク	Nat Commun / PubMed:37553324 / PubMed Central
手法	EM (単粒子)
解像度	2.2 - 3.4 Å
構造データ	36048 8j7t EMDB-36048, PDB-8j7t: Cryo-EM structure of hZnT7-Fab complex in zinc-unbound state, determined in outward-facing conformation 手法: EM (単粒子) / 解像度: 2.2 Å 36049 8j7u EMDB-36049, PDB-8j7u: Cryo-EM structure of hZnT7-Fab complex in zinc-bound state, determined in outward-facing conformation 手法: EM (単粒子) / 解像度: 3.12 Å 36050 8j7v EMDB-36050, PDB-8j7v: Cryo-EM structure of hZnT7-Fab complex in zinc-unbound state, determined in heterogeneous conformations- one subunit in an inward-facing and the other in an outward-facing conformation 手法: EM (単粒子) / 解像度: 2.79 Å 36051 8j7w EMDB-36051, PDB-8j7w: Cryo-EM structure of hZnT7-Fab complex in zinc state 2, determined in heterogeneous conformations- one subunit in an inward-facing zinc-bound and the other in an outward-facing zinc-bound conformation 手法: EM (単粒子) / 解像度: 2.92 Å 36052 8j7x EMDB-36052, PDB-8j7x: Cryo-EM structure of hZnT7DeltaHis-loop-Fab complex in zinc-unbound state, determined in outward-facing conformation 手法: EM (単粒子) / 解像度: 3.4 Å 36053 8j7y EMDB-36053, PDB-8j7y: Cryo-EM structure of hZnT7DeltaHis-loop-Fab complex in zinc-bound state, determined in outward-facing conformation 手法: EM (単粒子) / 解像度: 3.4 Å 36055 8j80 EMDB-36055, PDB-8j80: Cryo-EM structure of hZnT7-Fab complex in zinc state 1, determined in heterogeneous conformations- one subunit in an inward-facing zinc-bound and the other in an outward-facing zinc-unbound conformation 手法: EM (単粒子) / 解像度: 2.68 Å
化合物	ChemComp-ZN: Unknown entry
由来	homo sapiens (ヒト) mus musculus (ハツカネズミ)
キーワード	METAL TRANSPORT / zinc (亜鉛) / proton (陽子) / transporter (運搬体タンパク質) / Golgi apparatus (ゴルジ体) / metal transporter / histidine-rich loop