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-Structure paper
タイトル | An oligopeptide permease, OppABCD, requires an iron-sulfur cluster domain for functionality. |
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ジャーナル・号・ページ | Nat Struct Mol Biol, Year 2024 |
掲載日 | 2024年3月28日 |
著者 | Xiaolin Yang / Tianyu Hu / Jingxi Liang / Zhiqi Xiong / Zhenli Lin / Yao Zhao / Xiaoting Zhou / Yan Gao / Shan Sun / Xiuna Yang / Luke W Guddat / Haitao Yang / Zihe Rao / Bing Zhang / |
PubMed 要旨 | Oligopeptide permease, OppABCD, belongs to the type I ABC transporter family. Its role is to import oligopeptides into bacteria for nutrient uptake and to modulate the host immune response. OppABCD ...Oligopeptide permease, OppABCD, belongs to the type I ABC transporter family. Its role is to import oligopeptides into bacteria for nutrient uptake and to modulate the host immune response. OppABCD consists of a cluster C substrate-binding protein (SBP), OppA, membrane-spanning OppB and OppC subunits, and an ATPase, OppD, that contains two nucleotide-binding domains (NBDs). Here, using cryo-electron microscopy, we determined the high-resolution structures of Mycobacterium tuberculosis OppABCD in the resting state, oligopeptide-bound pre-translocation state, AMPPNP-bound pre-catalytic intermediate state and ATP-bound catalytic intermediate state. The structures show an assembly of a cluster C SBP with its ABC translocator and a functionally required [4Fe-4S] cluster-binding domain in OppD. Moreover, the ATP-bound OppABCD structure has an outward-occluded conformation, although no substrate was observed in the transmembrane cavity. Here, we reveal an oligopeptide recognition and translocation mechanism of OppABCD, which provides a perspective on how this and other type I ABC importers facilitate bulk substrate transfer across the lipid bilayer. |
リンク | Nat Struct Mol Biol / PubMed:38548954 |
手法 | EM (単粒子) / X線回折 |
解像度 | 1.98 - 3.28 Å |
構造データ | EMDB-35990, PDB-8j5q: EMDB-35991, PDB-8j5r: EMDB-35992, PDB-8j5s: EMDB-35993, PDB-8j5t: PDB-8j5u: |
化合物 | ChemComp-SF4: ChemComp-ANP: ChemComp-MG: ChemComp-ATP: ChemComp-HOH: |
由来 |
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キーワード | MEMBRANE PROTEIN (膜タンパク質) / OppABCD / Type I ABC importer / Oligopeptide permease / Mycobacterium tuberculosis (結核菌) / AMPPNP-Mg / Pre-catalytic intermediate state / ATP-Mg / Occluded state / PEPTIDE BINDING PROTEIN / OppA / Oligopeptide binding protein / Cluster C SBP |