EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Affinity-matured homotypic interactions induce spectrum of PfCSP structures that influence protection from malaria infection.
ジャーナル・号・ページ	Nat Commun, Vol. 14, Issue 1, Page 4546, Year 2023
掲載日	2023年7月28日
著者	Gregory M Martin / Jonathan L Torres / Tossapol Pholcharee / David Oyen / Yevel Flores-Garcia / Grace Gibson / Re'em Moskovitz / Nathan Beutler / Diana D Jung / Jeffrey Copps / Wen-Hsin Lee / Gonzalo Gonzalez-Paez / Daniel Emerling / Randall S MacGill / Emily Locke / C Richter King / Fidel Zavala / Ian A Wilson / Andrew B Ward /
PubMed 要旨	The generation of high-quality antibody responses to Plasmodium falciparum (Pf) circumsporozoite protein (PfCSP), the primary surface antigen of Pf sporozoites, is paramount to the development of an ...The generation of high-quality antibody responses to Plasmodium falciparum (Pf) circumsporozoite protein (PfCSP), the primary surface antigen of Pf sporozoites, is paramount to the development of an effective malaria vaccine. Here we present an in-depth structural and functional analysis of a panel of potent antibodies encoded by the immunoglobulin heavy chain variable (IGHV) gene IGHV3-33, which is among the most prevalent and potent antibody families induced in the anti-PfCSP immune response and targets the Asn-Ala-Asn-Pro (NANP) repeat region. Cryo-electron microscopy (cryo-EM) reveals a remarkable spectrum of helical antibody-PfCSP structures stabilized by homotypic interactions between tightly packed fragments antigen binding (Fabs), many of which correlate with somatic hypermutation. We demonstrate a key role of these mutated homotypic contacts for high avidity binding to PfCSP and in protection from Pf malaria infection. Together, these data emphasize the importance of anti-homotypic affinity maturation in the frequent selection of IGHV3-33 antibodies and highlight key features underlying the potent protection of this antibody family.
リンク	Nat Commun / PubMed:37507365 / PubMed Central
手法	EM (単粒子) / X線回折
解像度	1.9 - 3.84 Å
構造データ	27781 8dyt EMDB-27781, PDB-8dyt: Cryo-EM structure of 227 Fab in complex with (NPNA)8 peptide 手法: EM (単粒子) / 解像度: 3.3 Å 27784 8dyw EMDB-27784, PDB-8dyw: Cryo-EM structure of 239 Fab in complex with recombinant shortened Plasmodium falciparum circumsporozoite protein (rsCSP) 手法: EM (単粒子) / 解像度: 3.72 Å 27785 8dyx EMDB-27785, PDB-8dyx: Cryo-EM structure of 311 Fab in complex with recombinant shortened Plasmodium falciparum circumsporozoite protein (rsCSP) 手法: EM (単粒子) / 解像度: 3.0 Å 27786 8dyy EMDB-27786, PDB-8dyy: Cryo-EM structure of 334 Fab in complex with recombinant shortened Plasmodium falciparum circumsporozoite protein (rsCSP) 手法: EM (単粒子) / 解像度: 3.62 Å 27787 8dz3 EMDB-27787, PDB-8dz3: Cryo-EM structure of 337 Fab in complex with recombinant shortened Plasmodium falciparum circumsporozoite protein (rsCSP) 手法: EM (単粒子) / 解像度: 2.7 Å 27788 8dz4 EMDB-27788, PDB-8dz4: Cryo-EM structure of 356 Fab in complex with recombinant shortened Plasmodium falciparum circumsporozoite protein (rsCSP) 手法: EM (単粒子) / 解像度: 3.2 Å 27789 8dz5 EMDB-27789, PDB-8dz5: Cryo-EM structure of 364 Fab in complex with recombinant shortened Plasmodium falciparum circumsporozoite protein (rsCSP) 手法: EM (単粒子) / 解像度: 3.84 Å PDB-8ekf: X-ray crystal structure of 311R Fab in complex with the PfCSP peptide NPNA-3 手法: X-RAY DIFFRACTION / 解像度: 1.9 Å
化合物	ChemComp-HOH: WATER / 水
由来	plasmodium falciparum (マラリア病原虫) homo sapiens (ヒト) plasmodium falciparum 3d7 (マラリア病原虫) Escherichia coli (大腸菌)
キーワード	IMMUNE SYSTEM (免疫系) / malaria antibody / PfCSP / Antibody (抗体) / Antigen (抗原) / Malaria (マラリア)