EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	DNA-binding mechanism and evolution of replication protein A.
ジャーナル・号・ページ	Nat Commun, Vol. 14, Issue 1, Page 2326, Year 2023
掲載日	2023年4月22日
著者	Clément Madru / Markel Martínez-Carranza / Sébastien Laurent / Alessandra C Alberti / Maelenn Chevreuil / Bertrand Raynal / Ahmed Haouz / Rémy A Le Meur / Marc Delarue / Ghislaine Henneke / Didier Flament / Mart Krupovic / Pierre Legrand / Ludovic Sauguet /
PubMed 要旨	Replication Protein A (RPA) is a heterotrimeric single stranded DNA-binding protein with essential roles in DNA replication, recombination and repair. Little is known about the structure of RPA in ...Replication Protein A (RPA) is a heterotrimeric single stranded DNA-binding protein with essential roles in DNA replication, recombination and repair. Little is known about the structure of RPA in Archaea, the third domain of life. By using an integrative structural, biochemical and biophysical approach, we extensively characterize RPA from Pyrococcus abyssi in the presence and absence of DNA. The obtained X-ray and cryo-EM structures reveal that the trimerization core and interactions promoting RPA clustering on ssDNA are shared between archaea and eukaryotes. However, we also identified a helical domain named AROD (Acidic Rpa1 OB-binding Domain), and showed that, in Archaea, RPA forms an unanticipated tetrameric supercomplex in the absence of DNA. The four RPA molecules clustered within the tetramer could efficiently coat and protect stretches of ssDNA created by the advancing replisome. Finally, our results provide insights into the evolution of this primordial replication factor in eukaryotes.
リンク	Nat Commun / PubMed:37087464 / PubMed Central
手法	EM (単粒子) / X線回折
解像度	1.8 - 8.24 Å
構造データ	16444 8c5y EMDB-16444, PDB-8c5y: RPA tetrameric supercomplex from Pyrococcus abyssi 手法: EM (単粒子) / 解像度: 3.35 Å 16445 8c5z EMDB-16445, PDB-8c5z: RPA tetrameric supercomplex with AROD-OB-1 手法: EM (単粒子) / 解像度: 3.8 Å EMDB-16448: Replication Protein A bound to DNA 手法: EM (単粒子) / 解像度: 3.9 Å 16826 8oej EMDB-16826, PDB-8oej: Extended RPA-DNA nucleoprotein filament 手法: EM (単粒子) / 解像度: 8.0 Å 16827 8oel EMDB-16827, PDB-8oel: Condensed RPA-DNA nucleoprotein filament 手法: EM (単粒子) / 解像度: 8.2 Å PDB-8aa9: Crystal structure of the Rpa1 AROD-OB-1 domains 手法: X-RAY DIFFRACTION / 解像度: 1.8 Å PDB-8aaj: Crystal structure of the Pyrococcus abyssi RPA (apo form) 手法: X-RAY DIFFRACTION / 解像度: 3.7 Å PDB-8aas: Crystal structure of the Pyrococcus abyssi RPA trimerization core bound to poly-dT20 ssDNA 手法: X-RAY DIFFRACTION / 解像度: 3.2 Å
化合物	ChemComp-PEG: DI(HYDROXYETHYL)ETHER / ジエチレングリコ-ル / ジエチレングリコール ChemComp-CL: Unknown entry / クロリド / 塩化物 ChemComp-HOH: WATER / 水 ChemComp-ZN: Unknown entry ChemComp-SO4: SULFATE ION / 硫酸ジアニオン / 硫酸塩 ChemComp-CA: Unknown entry / カルシウムジカチオン
由来	pyrococcus abyssi (古細菌) escherichia coli (大腸菌) pyrococcus abyssi ge5 (古細菌) synthetic construct (人工物)
キーワード	DNA BINDING PROTEIN (DNA結合タンパク質) / Replication protein A (DNA複製) / ssDNA-Binding protein / DNA replication (DNA複製) / single strand DNA-binding protein / RPA