EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural insights into RNA-mediated transcription regulation in bacteria.
ジャーナル・号・ページ	Mol Cell, Vol. 82, Issue 20, Page 3885-3900.e10, Year 2022
掲載日	2022年10月20日
著者	Sanjay Dey / Claire Batisse / Jinal Shukla / Michael W Webster / Maria Takacs / Charlotte Saint-André / Albert Weixlbaumer /
PubMed 要旨	RNA can regulate its own synthesis without auxiliary proteins. For example, U-rich RNA sequences signal RNA polymerase (RNAP) to pause transcription and are required for transcript release at ...RNA can regulate its own synthesis without auxiliary proteins. For example, U-rich RNA sequences signal RNA polymerase (RNAP) to pause transcription and are required for transcript release at intrinsic terminators in all kingdoms of life. In contrast, the regulatory RNA putL suppresses pausing and termination in cis. However, how nascent RNA modulates its own synthesis remains largely unknown. We present cryo-EM reconstructions of RNAP captured during transcription of putL variants or an unrelated sequence at a U-rich pause site. Our results suggest how putL suppresses pausing and promotes its synthesis. We demonstrate that transcribing a U-rich sequence, a ubiquitous trigger of intrinsic termination, promotes widening of the RNAP nucleic-acid-binding channel. Widening destabilizes RNAP interactions with DNA and RNA to facilitate transcript dissociation reminiscent of intrinsic transcription termination. Surprisingly, RNAP remains bound to DNA after transcript release. Our results provide the structural framework to understand RNA-mediated intrinsic transcription termination.
リンク	Mol Cell / PubMed:36220101
手法	EM (単粒子)
解像度	3.4 - 4.5 Å
構造データ	15327 8aby EMDB-15327, PDB-8aby: RNA polymerase bound to purified in vitro transcribed regulatory RNA putL - pause prone, closed clamp state 手法: EM (単粒子) / 解像度: 3.7 Å 15328 8abz EMDB-15328, PDB-8abz: RNA polymerase at U-rich pause bound to non-regulatory RNA - pause prone, closed clamp state 手法: EM (単粒子) / 解像度: 3.4 Å 15329 8ac0 EMDB-15329, PDB-8ac0: RNA polymerase at U-rich pause bound to regulatory RNA putL - active, closed clamp state 手法: EM (単粒子) / 解像度: 4.1 Å 15330 8ac1 EMDB-15330, PDB-8ac1: RNA polymerase at U-rich pause bound to non-regulatory RNA - inactive, open clamp state 手法: EM (単粒子) / 解像度: 4.06 Å 15331 8ac2 EMDB-15331, PDB-8ac2: RNA polymerase- post-terminated, open clamp state 手法: EM (単粒子) / 解像度: 3.7 Å 15352 8acp EMDB-15352, PDB-8acp: RNA polymerase at U-rich pause bound to regulatory RNA putL - inactive, open clamp state 手法: EM (単粒子) / 解像度: 4.5 Å 15357 8ad1 EMDB-15357, PDB-8ad1: RNA polymerase at U-rich pause bound to RNA putL triple mutant - pause prone, closed clamp state 手法: EM (単粒子) / 解像度: 4.1 Å EMDB-15612: RNA polymerase at U-rich pause bound to regulatory RNA putL - Pause-prone, closed clamp state 手法: EM (単粒子) / 解像度: 4.28 Å EMDB-15613: RNA polymerase bound to purified in vitro transcribed regulatory RNA putL - inactive, open clamp state 手法: EM (単粒子) / 解像度: 4.4 Å
化合物	ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-ZN: Unknown entry
由来	escherichia coli k-12 (大腸菌) escherichia coli (大腸菌) escherichia coli bl21 (大腸菌)
キーワード	TRANSCRIPTION (転写 (生物学)) / RNA polymerase (RNAポリメラーゼ) / transcriptional pausing / transcription termination / regulatory RNA (RNA干渉)