EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Cryo-EM structures of the Synechocystis sp. PCC 6803 cytochrome b6f complex with and without the regulatory PetP subunit.
ジャーナル・号・ページ	Biochem J, Vol. 479, Issue 13, Page 1487-1503, Year 2022
掲載日	2022年7月15日
著者	Matthew S Proctor / Lorna A Malone / David A Farmer / David J K Swainsbury / Frederick R Hawkings / Federica Pastorelli / Thomas Z Emrich-Mills / C Alistair Siebert / C Neil Hunter / Matthew P Johnson / Andrew Hitchcock /
PubMed 要旨	In oxygenic photosynthesis, the cytochrome b6f (cytb6f) complex links the linear electron transfer (LET) reactions occurring at photosystems I and II and generates a transmembrane proton gradient via ...In oxygenic photosynthesis, the cytochrome b6f (cytb6f) complex links the linear electron transfer (LET) reactions occurring at photosystems I and II and generates a transmembrane proton gradient via the Q-cycle. In addition to this central role in LET, cytb6f also participates in a range of processes including cyclic electron transfer (CET), state transitions and photosynthetic control. Many of the regulatory roles of cytb6f are facilitated by auxiliary proteins that differ depending upon the species, yet because of their weak and transient nature the structural details of these interactions remain unknown. An apparent key player in the regulatory balance between LET and CET in cyanobacteria is PetP, a ∼10 kDa protein that is also found in red algae but not in green algae and plants. Here, we used cryogenic electron microscopy to determine the structure of the Synechocystis sp. PCC 6803 cytb6f complex in the presence and absence of PetP. Our structures show that PetP interacts with the cytoplasmic side of cytb6f, displacing the C-terminus of the PetG subunit and shielding the C-terminus of cytochrome b6, which binds the heme cn cofactor that is suggested to mediate CET. The structures also highlight key differences in the mode of plastoquinone binding between cyanobacterial and plant cytb6f complexes, which we suggest may reflect the unique combination of photosynthetic and respiratory electron transfer in cyanobacterial thylakoid membranes. The structure of cytb6f from a model cyanobacterial species amenable to genetic engineering will enhance future site-directed mutagenesis studies of structure-function relationships in this crucial ET complex.
リンク	Biochem J / PubMed:35726684 / PubMed Central
手法	EM (単粒子)
解像度	2.8 - 3.15 Å
構造データ	14224 7r0w EMDB-14224, PDB-7r0w: 2.8 Angstrom cryo-EM structure of the dimeric cytochrome b6f-PetP complex from Synechocystis sp. PCC 6803 with natively bound lipids and plastoquinone molecules 手法: EM (単粒子) / 解像度: 2.8 Å 15017 7zxy EMDB-15017, PDB-7zxy: 3.15 Angstrom cryo-EM structure of the dimeric cytochrome b6f complex from Synechocystis sp. PCC 6803 with natively bound plastoquinone and lipid molecules. 手法: EM (単粒子) / 解像度: 3.15 Å
化合物	ChemComp-HEM: PROTOPORPHYRIN IX CONTAINING FE / Heme B ChemComp-PGV: (1R)-2-{[{[(2S)-2,3-DIHYDROXYPROPYL]OXY}(HYDROXY)PHOSPHORYL]OXY}-1-[(PALMITOYLOXY)METHYL]ETHYL (11E)-OCTADEC-11-ENOATE / リン脂質YM / Phosphatidylglycerol ChemComp-ECH: beta,beta-caroten-4-one / (9′Z)-β-エキネノン / Echinenone ChemComp-PL9: 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE / プラストキノン ChemComp-CLA: CHLOROPHYLL A / クロロフィルa ChemComp-HEC: HEME C / Heme C ChemComp-LMG: 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE ChemComp-6PL: (4S,7R)-4-HYDROXY-N,N,N-TRIMETHYL-9-OXO-7-[(PALMITOYLOXY)METHYL]-3,5,8-TRIOXA-4-PHOSPHAHEXACOSAN-1-AMINIUM 4-OXIDE / O-(1-O-パルミトイル-2-O-ステアロイル-L-グリセロ-3-ホスホ)コリン / リン脂質YM ChemComp-2WA: (1S,8E)-1-{[(2S)-1-hydroxy-3-{[(1S)-1-hydroxypentadecyl]oxy}propan-2-yl]oxy}heptadec-8-en-1-ol ChemComp-FES: FE2/S2 (INORGANIC) CLUSTER / 鉄・硫黄クラスター ChemComp-SQD: 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL / (2S)-1-O,2-O-ジパルミトイル-3-O-(6-スルホ-6-デオキシ-α-D-グルコピラノシル)グリセロ-ル ChemComp-LFA: EICOSANE / イコサン / エイコサン ChemComp-HOH: WATER / 水
由来	synechocystis sp. pcc 6803 (バクテリア) synechocystis (バクテリア)
キーワード	PHOTOSYNTHESIS (光合成) / Complex / cytochrome (シトクロム) / membrane protein (膜タンパク質) / electron transport (電子伝達系) / OXIDOREDUCTASE (酸化還元酵素) / cytochrome bc complexes / electron transfer (電子移動反応) / cytochrome b6f (シトクロムb6f複合体) / cyanobacteria (藍藻)