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-Structure paper
タイトル | Cryo-electron microscopy and image classification reveal the existence and structure of the coxsackievirus A6 virion. |
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ジャーナル・号・ページ | Commun Biol, Vol. 5, Issue 1, Page 898, Year 2022 |
掲載日 | 2022年9月2日 |
著者 | Carina R Büttner / Radovan Spurný / Tibor Füzik / Pavel Plevka / |
PubMed 要旨 | Coxsackievirus A6 (CV-A6) has recently overtaken enterovirus A71 and CV-A16 as the primary causative agent of hand, foot, and mouth disease worldwide. Virions of CV-A6 were not identified in previous ...Coxsackievirus A6 (CV-A6) has recently overtaken enterovirus A71 and CV-A16 as the primary causative agent of hand, foot, and mouth disease worldwide. Virions of CV-A6 were not identified in previous structural studies, and it was speculated that the virus is unique among enteroviruses in using altered particles with expanded capsids to infect cells. In contrast, the virions of other enteroviruses are required for infection. Here we used cryo-electron microscopy (cryo-EM) to determine the structures of the CV-A6 virion, altered particle, and empty capsid. We show that the CV-A6 virion has features characteristic of virions of other enteroviruses, including a compact capsid, VP4 attached to the inner capsid surface, and fatty acid-like molecules occupying the hydrophobic pockets in VP1 subunits. Furthermore, we found that in a purified sample of CV-A6, the ratio of infectious units to virions is 1 to 500. Therefore, it is likely that virions of CV-A6 initiate infection, like those of other enteroviruses. Our results provide evidence that future vaccines against CV-A6 should target its virions instead of the antigenically distinct altered particles. Furthermore, the structure of the virion provides the basis for the rational development of capsid-binding inhibitors that block the genome release of CV-A6. |
リンク | Commun Biol / PubMed:36056184 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 2.5 - 2.82 Å |
構造データ | EMDB-14183, PDB-7qvx: EMDB-14184, PDB-7qvy: EMDB-14186, PDB-7qw9: |
化合物 | ChemComp-STE: ChemComp-MYR: |
由来 |
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キーワード | VIRUS (ウイルス) / enterovirus (エンテロウイルス) / coxsackievirus A6 / altered particle / capsid (カプシド) / cryo-EM (低温電子顕微鏡法) / empty particle / virion (ウイルス) / native |