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-Structure paper
タイトル | Analysis of the mechanosensor channel functionality of TACAN. |
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ジャーナル・号・ページ | Elife, Vol. 10, Year 2021 |
掲載日 | 2021年8月10日 |
著者 | Yiming Niu / Xiao Tao / George Vaisey / Paul Dominic B Olinares / Hanan Alwaseem / Brian T Chait / Roderick MacKinnon / |
PubMed 要旨 | Mechanosensitive ion channels mediate transmembrane ion currents activated by mechanical forces. A mechanosensitive ion channel called TACAN was recently reported. We began to study TACAN with the ...Mechanosensitive ion channels mediate transmembrane ion currents activated by mechanical forces. A mechanosensitive ion channel called TACAN was recently reported. We began to study TACAN with the intent to understand how it senses mechanical forces and functions as an ion channel. Using cellular patch-recording methods, we failed to identify mechanosensitive ion channel activity. Using membrane reconstitution methods, we found that TACAN, at high protein concentrations, produces heterogeneous conduction levels that are not mechanosensitive and are most consistent with disruptions of the lipid bilayer. We determined the structure of TACAN using single-particle cryo-electron microscopy and observed that it is a symmetrical dimeric transmembrane protein. Each protomer contains an intracellular-facing cleft with a coenzyme A cofactor, confirmed by mass spectrometry. The TACAN protomer is related in three-dimensional structure to a fatty acid elongase, ELOVL7. Whilst its physiological function remains unclear, we anticipate that TACAN is not a mechanosensitive ion channel. |
リンク | Elife / PubMed:34374644 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 2.8 - 3.5 Å |
構造データ | EMDB-24107, PDB-7n0k: EMDB-24108, PDB-7n0l: |
化合物 | ChemComp-COA: |
由来 |
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キーワード | MEMBRANE PROTEIN (膜タンパク質) / lipid metabolism (脂質代謝) / coenzyme A (補酵素A) |