EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Hippocampal AMPA receptor assemblies and mechanism of allosteric inhibition.
ジャーナル・号・ページ	Nature, Vol. 594, Issue 7863, Page 448-453, Year 2021
掲載日	2021年5月12日
著者	Jie Yu / Prashant Rao / Sarah Clark / Jaba Mitra / Taekjip Ha / Eric Gouaux /
PubMed 要旨	AMPA-selective glutamate receptors mediate the transduction of signals between the neuronal circuits of the hippocampus. The trafficking, localization, kinetics and pharmacology of AMPA receptors are ...AMPA-selective glutamate receptors mediate the transduction of signals between the neuronal circuits of the hippocampus. The trafficking, localization, kinetics and pharmacology of AMPA receptors are tuned by an ensemble of auxiliary protein subunits, which are integral membrane proteins that associate with the receptor to yield bona fide receptor signalling complexes. Thus far, extensive studies of recombinant AMPA receptor-auxiliary subunit complexes using engineered protein constructs have not been able to faithfully elucidate the molecular architecture of hippocampal AMPA receptor complexes. Here we obtain mouse hippocampal, calcium-impermeable AMPA receptor complexes using immunoaffinity purification and use single-molecule fluorescence and cryo-electron microscopy experiments to elucidate three major AMPA receptor-auxiliary subunit complexes. The GluA1-GluA2, GluA1-GluA2-GluA3 and GluA2-GluA3 receptors are the predominant assemblies, with the auxiliary subunits TARP-γ8 and CNIH2-SynDIG4 non-stochastically positioned at the B'/D' and A'/C' positions, respectively. We further demonstrate how the receptor-TARP-γ8 stoichiometry explains the mechanism of and submaximal inhibition by a clinically relevant, brain-region-specific allosteric inhibitor.
リンク	Nature / PubMed:33981040 / PubMed Central
手法	EM (単粒子)
解像度	3.25 - 7.5 Å
構造データ	23283 7ldd EMDB-23283, PDB-7ldd: native AMPA receptor 手法: EM (単粒子) / 解像度: 3.4 Å 23284 7lde EMDB-23284, PDB-7lde: native AMPA receptor 手法: EM (単粒子) / 解像度: 3.9 Å EMDB-23285: native AMPA receptor 手法: EM (単粒子) / 解像度: 4.1 Å EMDB-23286: native AMPA receptor 手法: EM (単粒子) / 解像度: 3.9 Å EMDB-23287: native AMPA receptor 手法: EM (単粒子) / 解像度: 3.9 Å EMDB-23288: native AMPA receptor 手法: EM (単粒子) / 解像度: 6.5 Å EMDB-23289: native AMPA receptor 手法: EM (単粒子) / 解像度: 5.1 Å EMDB-23290: native AMPA receptor 手法: EM (単粒子) / 解像度: 7.5 Å 23292 7lep EMDB-23292, PDB-7lep: The composite LBD-TMD structure combined from all hippocampal AMPAR subtypes at 3.25 Angstrom resolution 手法: EM (単粒子) / 解像度: 3.25 Å
化合物	ChemComp-ZK1: {[7-morpholin-4-yl-2,3-dioxo-6-(trifluoromethyl)-3,4-dihydroquinoxalin-1(2H)-yl]methyl}phosphonic acid / ZK-200775 / アンタゴニスト, 薬剤YM / Fanapanel ChemComp-R16: HEXADECANE / ヘキサデカン / ヘキサデカン ChemComp-OCT: N-OCTANE / オクタン / オクタン ChemComp-HP6: HEPTANE / ヘプタン-1,1,1-トリイルラジカル / Heptane ChemComp-D10: DECANE / デカン / デカン ChemComp-NAG: 2-acetamido-2-deoxy-beta-D-glucopyranose / N-アセチル-β-D-グルコサミン / N-アセチルグルコサミン ChemComp-D12: DODECANE / ドデカン / ドデカン ChemComp-C14: TETRADECANE / テトラデカン / テトラデカン ChemComp-DD9: nonane / ノナン / ノナン ChemComp-XVD: 6-[2-chloro-6-(trifluoromethoxy)phenyl]-1H-benzimidazol-2-ol ChemComp-POV: (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate / リン脂質YM / POホスファチジルコリン
由来	mus musculus (ハツカネズミ) Mouse (ネズミ)
キーワード	MEMBRANE PROTEIN/IMMUNE SYSTEM (生体膜) / neurotransmitter (神経伝達物質) / two-fold symmetry / hippocampus / ion-channel (イオンチャネル) / MEMBRANE PROTEIN (膜タンパク質) / SIGNALING PROTEIN-IMMUNE SYSTEM complex / MEMBRANE PROTEIN-IMMUNE SYSTEM complex (生体膜) / glycosylation (グリコシル化) / Native hippocampal ion channel