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-Structure paper
タイトル | Cryo-EM structure of the mature and infective Mayaro virus at 4.4 Å resolution reveals features of arthritogenic alphaviruses. |
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ジャーナル・号・ページ | Nat Commun, Vol. 12, Issue 1, Page 3038, Year 2021 |
掲載日 | 2021年5月24日 |
著者 | Helder V Ribeiro-Filho / Lais D Coimbra / Alexandre Cassago / Rebeca P F Rocha / João Victor da Silva Guerra / Rafael de Felicio / Carolina Moretto Carnieli / Luiza Leme / Antonio Cláudio Padilha / Adriana F Paes Leme / Daniela B B Trivella / Rodrigo Villares Portugal / Paulo Sérgio Lopes-de-Oliveira / Rafael Elias Marques / |
PubMed 要旨 | Mayaro virus (MAYV) is an emerging arbovirus of the Americas that may cause a debilitating arthritogenic disease. The biology of MAYV is not fully understood and largely inferred from related ...Mayaro virus (MAYV) is an emerging arbovirus of the Americas that may cause a debilitating arthritogenic disease. The biology of MAYV is not fully understood and largely inferred from related arthritogenic alphaviruses. Here, we present the structure of MAYV at 4.4 Å resolution, obtained from a preparation of mature, infective virions. MAYV presents typical alphavirus features and organization. Interactions between viral proteins that lead to particle formation are described together with a hydrophobic pocket formed between E1 and E2 spike proteins and conformational epitopes specific of MAYV. We also describe MAYV glycosylation residues in E1 and E2 that may affect MXRA8 host receptor binding, and a molecular "handshake" between MAYV spikes formed by N262 glycosylation in adjacent E2 proteins. The structure of MAYV is suggestive of structural and functional complexity among alphaviruses, which may be targeted for specificity or antiviral activity. |
リンク | Nat Commun / PubMed:34031424 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 4.4 Å |
構造データ | EMDB-22961, PDB-7ko8: |
由来 |
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キーワード | VIRUS (ウイルス) / alphavirus / mayaro virus / single particle cryo-EM / mature and infective viral particle |