EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Mechanism of membrane-tethered mitochondrial protein synthesis.
ジャーナル・号・ページ	Science, Vol. 371, Issue 6531, Page 846-849, Year 2021
掲載日	2021年2月19日
著者	Yuzuru Itoh / Juni Andréll / Austin Choi / Uwe Richter / Priyanka Maiti / Robert B Best / Antoni Barrientos / Brendan J Battersby / Alexey Amunts /
PubMed 要旨	Mitochondrial ribosomes (mitoribosomes) are tethered to the mitochondrial inner membrane to facilitate the cotranslational membrane insertion of the synthesized proteins. We report cryo-electron ...Mitochondrial ribosomes (mitoribosomes) are tethered to the mitochondrial inner membrane to facilitate the cotranslational membrane insertion of the synthesized proteins. We report cryo-electron microscopy structures of human mitoribosomes with nascent polypeptide, bound to the insertase oxidase assembly 1-like (OXA1L) through three distinct contact sites. OXA1L binding is correlated with a series of conformational changes in the mitoribosomal large subunit that catalyze the delivery of newly synthesized polypeptides. The mechanism relies on the folding of mL45 inside the exit tunnel, forming two specific constriction sites that would limit helix formation of the nascent chain. A gap is formed between the exit and the membrane, making the newly synthesized proteins accessible. Our data elucidate the basis by which mitoribosomes interact with the OXA1L insertase to couple protein synthesis and membrane delivery.
リンク	Science / PubMed:33602856 / PubMed Central
手法	EM (単粒子)
解像度	2.59 - 3.2 Å
構造データ	11278 6zm5 EMDB-11278, PDB-6zm5: Human mitochondrial ribosome in complex with OXA1L, mRNA, A/A tRNA, P/P tRNA and nascent polypeptide 手法: EM (単粒子) / 解像度: 2.89 Å 11279 6zm6 EMDB-11279, PDB-6zm6: Human mitochondrial ribosome in complex with mRNA, A/A tRNA and P/P tRNA 手法: EM (単粒子) / 解像度: 2.59 Å EMDB-11280: Human mitochondrial ribosome in complex with OXA1L, mRNA, A/A tRNA, P/P tRNA and nascent polypeptide, local-masked aligned on CP 手法: EM (単粒子) / 解像度: 3.2 Å EMDB-11281: Human mitochondrial ribosome in complex with OXA1L, mRNA, A/A tRNA, P/P tRNA and nascent polypeptide, local-masked aligned on L10-L7/L12-stalk 手法: EM (単粒子) / 解像度: 3.16 Å EMDB-11282: Human mitochondrial ribosome in complex with OXA1L, mRNA, A/A tRNA, P/P tRNA and nascent polypeptide, local-masked aligned on SSU body 手法: EM (単粒子) / 解像度: 3.03 Å EMDB-11283: Human mitochondrial ribosome in complex with OXA1L, mRNA, A/A tRNA, P/P tRNA and nascent polypeptide, local-masked aligned on SSU head 手法: EM (単粒子) / 解像度: 3.07 Å EMDB-11284: Human mitochondrial ribosome in complex with mRNA, A/A tRNA and P/P tRNA, local-masked aligned on CP 手法: EM (単粒子) / 解像度: 2.89 Å EMDB-11285: Human mitochondrial ribosome in complex with mRNA, A/A tRNA and P/P tRNA, local-masked aligned on L10-L7/L12-stalk 手法: EM (単粒子) / 解像度: 2.89 Å EMDB-11286: Human mitochondrial ribosome in complex with mRNA, A/A tRNA and P/P tRNA, local-masked aligned on SSU body 手法: EM (単粒子) / 解像度: 2.78 Å EMDB-11287: Human mitochondrial ribosome in complex with mRNA, A/A tRNA and P/P tRNA, local-masked aligned on SSU head. 手法: EM (単粒子) / 解像度: 2.84 Å
化合物	ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-K: Unknown entry / カリウムカチオン ChemComp-ZN: Unknown entry ChemComp-FES: FE2/S2 (INORGANIC) CLUSTER / 鉄・硫黄クラスター ChemComp-ATP: ADENOSINE-5'-TRIPHOSPHATE / ATP / ATP, エネルギー貯蔵分子YM / アデノシン三リン酸 ChemComp-GTP: GUANOSINE-5'-TRIPHOSPHATE / GTP / GTP, エネルギー貯蔵分子YM / グアノシン三リン酸 ChemComp-ALA: ALANINE / アラニン / アラニン ChemComp-HOH: WATER / 水
由来	homo sapiens (ヒト)
キーワード	RIBOSOME (リボソーム) / mitochondrion (ミトコンドリア) / translation (翻訳 (生物学)) / membrane insertion / translocon (トランスロコン) / peptidyl-tRNA (細菌の翻訳) / closed nascent-polypeptide tunnel