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-Structure paper
タイトル | Mechanistic Insights into Regulation of the ALC1 Remodeler by the Nucleosome Acidic Patch. |
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ジャーナル・号・ページ | Cell Rep, Vol. 33, Issue 12, Page 108529, Year 2020 |
掲載日 | 2020年12月22日 |
著者 | Laura C Lehmann / Luka Bacic / Graeme Hewitt / Klaus Brackmann / Anton Sabantsev / Guillaume Gaullier / Sofia Pytharopoulou / Gianluca Degliesposti / Hanneke Okkenhaug / Song Tan / Alessandro Costa / J Mark Skehel / Simon J Boulton / Sebastian Deindl / |
PubMed 要旨 | Upon DNA damage, the ALC1/CHD1L nucleosome remodeling enzyme (remodeler) is activated by binding to poly(ADP-ribose). How activated ALC1 recognizes the nucleosome, as well as how this recognition is ...Upon DNA damage, the ALC1/CHD1L nucleosome remodeling enzyme (remodeler) is activated by binding to poly(ADP-ribose). How activated ALC1 recognizes the nucleosome, as well as how this recognition is coupled to remodeling, is unknown. Here, we show that remodeling by ALC1 requires a wild-type acidic patch on the entry side of the nucleosome. The cryo-electron microscopy structure of a nucleosome-ALC1 linker complex reveals a regulatory linker segment that binds to the acidic patch. Mutations within this interface alter the dynamics of ALC1 recruitment to DNA damage and impede the ATPase and remodeling activities of ALC1. Full activation requires acidic patch-linker segment interactions that tether the remodeler to the nucleosome and couple ATP hydrolysis to nucleosome mobilization. Upon DNA damage, such a requirement may be used to modulate ALC1 activity via changes in the nucleosome acidic patches. |
リンク | Cell Rep / PubMed:33357431 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 2.5 - 3.0 Å |
構造データ | EMDB-11220, PDB-6zhx: EMDB-11221, PDB-6zhy: |
化合物 | ChemComp-HOH: |
由来 |
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キーワード | DNA BINDING PROTEIN (DNA結合タンパク質) / ALC1 (CHD1L) / CHD1L (CHD1L) / chromatin remodeler / DNA damage response / nucleosome (ヌクレオソーム) / NUCLEAR PROTEIN / GENE REGULATION (遺伝子発現の調節) / hexasome |