EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	A Structure-Based Model for the Complete Transcription Cycle of Influenza Polymerase.
ジャーナル・号・ページ	Cell, Vol. 181, Issue 4, Page 877-893.e21, Year 2020
掲載日	2020年5月14日
著者	Joanna M Wandzik / Tomas Kouba / Manikandan Karuppasamy / Alexander Pflug / Petra Drncova / Jan Provaznik / Nayara Azevedo / Stephen Cusack /
PubMed 要旨	Influenza polymerase uses unique mechanisms to synthesize capped and polyadenylated mRNAs from the genomic viral RNA (vRNA) template, which is packaged inside ribonucleoprotein particles (vRNPs). ...Influenza polymerase uses unique mechanisms to synthesize capped and polyadenylated mRNAs from the genomic viral RNA (vRNA) template, which is packaged inside ribonucleoprotein particles (vRNPs). Here, we visualize by cryoelectron microscopy the conformational dynamics of the polymerase during the complete transcription cycle from pre-initiation to termination, focusing on the template trajectory. After exiting the active site cavity, the template 3' extremity rebinds into a specific site on the polymerase surface. Here, it remains sequestered during all subsequent transcription steps, forcing the template to loop out as it further translocates. At termination, the strained connection between the bound template 5' end and the active site results in polyadenylation by stuttering at uridine 17. Upon product dissociation, further conformational changes release the trapped template, allowing recycling back into the pre-initiation state. Influenza polymerase thus performs transcription while tightly binding to and protecting both template ends, allowing efficient production of multiple mRNAs from a single vRNP.
リンク	Cell / PubMed:32304664
手法	EM (単粒子) / X線回折
解像度	2.41 - 3.52 Å
構造データ	10354 6szu EMDB-10354, PDB-6szu: Bat Influenza A polymerase pre-termination complex with pyrophosphate using 44-mer vRNA template with mutated oligo(U) sequence 手法: EM (単粒子) / 解像度: 2.41 Å 10355 6szv EMDB-10355, PDB-6szv: Bat Influenza A polymerase elongation complex with incoming UTP analogue (core + endonuclease only) 手法: EM (単粒子) / 解像度: 2.5 Å 10356 6t0n EMDB-10356, PDB-6t0n: Bat Influenza A polymerase pre-initiation complex 手法: EM (単粒子) / 解像度: 2.54 Å 10357 6t0r EMDB-10357, PDB-6t0r: Bat Influenza A polymerase product dissociation complex using 44-mer vRNA template with mutated oligo(U) sequence 手法: EM (単粒子) / 解像度: 2.82 Å 10358 6t0s EMDB-10358, PDB-6t0s: Bat Influenza A polymerase stuttering complex using 44-mer vRNA template with intact oligo(U) sequence 手法: EM (単粒子) / 解像度: 3.04 Å 10359 6t0u EMDB-10359, PDB-6t0u: Bat Influenza A polymerase product dissociation complex using 44-mer vRNA template with intact oligo(U) sequence 手法: EM (単粒子) / 解像度: 3.12 Å 10360 6t0v EMDB-10360, PDB-6t0v: Bat Influenza A polymerase elongation complex with incoming UTP analogue (complete polymerase) 手法: EM (単粒子) / 解像度: 3.02 Å 10361 6t0w EMDB-10361, PDB-6t0w: Human Influenza B polymerase recycling complex 手法: EM (単粒子) / 解像度: 3.18 Å 10368 6t2c EMDB-10368, PDB-6t2c: Bat Influenza A polymerase recycling complex 手法: EM (単粒子) / 解像度: 3.52 Å 10603 6tw1 EMDB-10603, PDB-6tw1: Bat Influenza A polymerase termination complex with pyrophosphate using 44-mer vRNA template with mutated oligo(U) sequence 手法: EM (単粒子) / 解像度: 2.7 Å PDB-6tu5: Influenza A/H7N9 polymerase core (apo) 手法: X-RAY DIFFRACTION / 解像度: 3.325 Å
化合物	ChemComp-M4H: 5-oxidanyl-4-oxidanylidene-1-[(1-pyrrolo[2,3-b]pyridin-1-ylcyclopentyl)methyl]pyridine-3-carboxylic acid / 1,4-ジヒドロ-5-ヒドロキシ-1-[1-(1H-ピロロ[2,3-b]ピリジン-1-イル)シクロペンタン-1-イルメ(以下略) ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-POP: PYROPHOSPHATE 2- / ピロリン酸塩 ChemComp-HOH: WATER / 水 ChemComp-2KH: 5'-O-[(S)-hydroxy{[(S)-hydroxy(phosphonooxy)phosphoryl]amino}phosphoryl]uridine
由来	influenza a virus (a/little yellow-shouldered bat/guatemala/060/2010(h17n10)) (A型インフルエンザウイルス) influenza b virus (B型インフルエンザウイルス) synthetic construct (人工物) influenza a virus (a/zhejiang/dtid-zju01/2013(h7n9)) (A型インフルエンザウイルス)
キーワード	VIRAL PROTEIN (ウイルスタンパク質) / Influenza (インフルエンザ) / polymerase (ポリメラーゼ) / viral transcription / RNA (リボ核酸) / RNA-dependent RNA polymerase (RNA依存性RNAポリメラーゼ)