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-Structure paper
タイトル | Architecture and subunit arrangement of native AMPA receptors elucidated by cryo-EM. |
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ジャーナル・号・ページ | Science, Vol. 364, Issue 6438, Page 355-362, Year 2019 |
掲載日 | 2019年4月26日 |
著者 | Yan Zhao / Shanshuang Chen / Adam C Swensen / Wei-Jun Qian / Eric Gouaux / |
PubMed 要旨 | Glutamate-gated AMPA receptors mediate the fast component of excitatory signal transduction at chemical synapses throughout all regions of the mammalian brain. AMPA receptors are tetrameric ...Glutamate-gated AMPA receptors mediate the fast component of excitatory signal transduction at chemical synapses throughout all regions of the mammalian brain. AMPA receptors are tetrameric assemblies composed of four subunits, GluA1-GluA4. Despite decades of study, the subunit composition, subunit arrangement, and molecular structure of native AMPA receptors remain unknown. Here we elucidate the structures of 10 distinct native AMPA receptor complexes by single-particle cryo-electron microscopy (cryo-EM). We find that receptor subunits are arranged nonstochastically, with the GluA2 subunit preferentially occupying the B and D positions of the tetramer and with triheteromeric assemblies comprising a major population of native AMPA receptors. Cryo-EM maps define the structure for S2-M4 linkers between the ligand-binding and transmembrane domains, suggesting how neurotransmitter binding is coupled to ion channel gating. |
リンク | Science / PubMed:30975770 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 4.5 - 32.0 Å |
構造データ | EMDB-0426: EMDB-0427: EMDB-0428: EMDB-0429: EMDB-0430: EMDB-0431: EMDB-0432: EMDB-20085: EMDB-9387, PDB-6njl: |
化合物 | ChemComp-ZK1: ChemComp-NAG: |
由来 |
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キーワード | MEMBRANE PROTEIN/IMMUNE SYSTEM (生体膜) / AMPA receptor (AMPA型グルタミン酸受容体) / ligand gated ion channel (リガンド依存性イオンチャネル) / neurotransmitter (神経伝達物質) / synapse (シナプス) / MEMBRANE PROTEIN (膜タンパク質) / MEMBRANE PROTEIN-IMMUNE SYSTEM complex (生体膜) |